Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Suitable for: WB, SDS-PAGE
製品名Native Human Vitronectin/S-Protein (Biotin)
Vitronectin/S-Protein タンパク質・ペプチド 製品一覧
精製度> 95 % SDS-PAGE.
ab92639 is prepared from fresh Human plasma using non-denaturing chromatography, then biotin labeled at primary amines. Purity is > 98% pure by SDS-PAGE.
タンパク質長Full length protein
Our Abpromise guarantee covers the use of ab92639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.82% Sodium phosphate, 0.58% Sodium chloride
- Complement S Protein
- S Protein
機能Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
配列類似性Contains 4 hemopexin repeats.
Contains 1 SMB (somatomedin-B) domain.
ドメインThe SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
翻訳後修飾Sulfated on 2 tyrosine residues.
N- and O-glycosylated.
Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
Phosphorylation sites are present in the extracellular medium.
細胞内局在Secreted, extracellular space.
- Information by UniProt
Lane 1: Vitronectin/S-Protein(2 ug) Reduced
Lane 2: Vitronectin/S-Protein (2 ug) Non-Reduced
Lane 3: Prestained Standard
Lane 1 : anti-Vitronectin/S-ProteinAvidin-AP at 1/3000 dilution
Lane 2 : anti-Vitronectin/S-Protein Avidin-AP at 1/3000 dilution
Lane 1 : ab92639 (Reduced)
Lane 2 : ab92639 (Non-reduced)
Lysates/proteins at 2 µg per lane.
Observed band size: 75 kDa why is the actual band size different from the predicted?
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.