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  1. Link

    native-human-serum-albumin-protein-biotin-ab8033.pdf

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Cardiovascular Blood Serum Proteins
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Native Human Serum Albumin protein (Biotin) (ab8033)

  • Datasheet
  • SDS
Submit a review Q&A (6)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Dot Blot - Native Human Serum Albumin protein (Biotin) (ab8033)

    Key features and details

    • Expression system: Native
    • Suitable for: Dot blot, ELISA, Immunomicroscopy, WB

    こちらの製品もご検討ください

    ELISA
    Product image
    Human Albumin ELISA Kit (ab179887)
    タンパク質
    Native Mouse Albumin protein (Azide free) (ab183228)
    一次抗体
    Anti-Mouse Serum Albumin antibody (ab19196)

    関連製品

    製品の詳細

    • 製品名

      Native Human Serum Albumin protein (Biotin)
      Human Serum Albumin タンパク質・ペプチド 製品一覧
    • 発現系

      Native
    • アクセッション番号

      P02768
    • タンパク質長

      Full length protein
    • Animal free

      No
    • 由来

      Native
      • 生物種

        Human
      • 予測される分子量

        69 kDa
    • 標識

      Biotin
    • 製品の詳細

      Native Human Human Serum Albumin protein (Biotin)

    特性

    Our Abpromise guarantee covers the use of ab8033 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      Dot blot

      ELISA

      Immunomicroscopy

      Western blot

    • 製品の状態

      Lyophilized
    • 備考

      Disclaimer Note-Human
      No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.

      Purification Technique:

      This product was prepared from normal serum delipidation a multi-stage process including selective precipitation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Human Albumin and anti-Human Serum.

    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride

    • 再構成
      Restore with 1.0 ml of deionized water (or equivalent).aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration. Stabiliser: 10 mg/ml Polyethylene Glycol (PEG-8000) Preservative: 0.01% (w/v) Sodium Azide

    関連情報

    • 別名

      • alb
      • ALBU_HUMAN
      • Albumin
      • Albumin (32 AA)
      • Albumin (AA 34)
      • Analbuminemia
      • Bisalbuminemia
      • Cell growth inhibiting protein 42
      • DKFZp779N1935
      • Dysalbuminemic hyperthyroxinemia
      • Growth inhibiting protein 20
      • HSA
      • Hyperthyroxinemia dysalbuminemic
      • PRO0883
      • PRO0903
      • PRO1341
      • Serum albumin
      see all
    • 機能

      Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
    • 組織特異性

      Plasma.
    • 関連疾患

      Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
    • 配列類似性

      Belongs to the ALB/AFP/VDB family.
      Contains 3 albumin domains.
    • 翻訳後修飾

      Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
      Glycated in diabetic patients.
      Phosphorylation sites are present in the extracelllular medium.
      Acetylated on Lys-223 by acetylsalicylic acid.
    • 細胞内局在

      Secreted.
    • Target information above from: UniProt accession P02768 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    画像

    • Dot Blot - Native Human Serum Albumin protein (Biotin) (ab8033)
      Dot Blot - Native Human Serum Albumin protein (Biotin) (ab8033)

      Dot Blot - Native Human Serum Albumin protein (Biotin) (ab8033).
      A three-fold serial dilution of ab8033 starting at 200ng was spotted onto 0.45 µm nitrocellulose and blocked in 1% BSA-TTBS for 30 min at 20°C. An HRP conjugated Streptavidin was incubated at 1/40,000 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc® 4000 MP.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (0)

    ab8033 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab8033 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-6 of 6 Abreviews or Q&A

    Question

    What is the spacer arm for the biotin conjugation?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 02 2012

    Answer

    Thank you for contacting us.

    The spacer arm is biotin-epsilon-aminocaproic acid (BAC). Here is a URL with a picture of it:

    http://www.chemicalbook.com/ProductChemicalPropertiesCB2753674_EN.htm

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Apr 02 2012

    Question

    What is the ratio of biotin to albumin?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 12 2012

    Answer

    Thank you for contacting Abcam.

    The ratio for this particular lot is 2.5 moles of biotin per mole of human albumin.
    Please tell us if we can provide more help with this issue.

    Read More

    Abcam Scientific Support

    Answered on Mar 12 2012

    Question

    What is the ratio of biotin to protein?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 20 2012

    Answer

    Thank you for contacting Abcam regarding ab8033.


    I have confirmed with the laboratory that our target biotin to protein ratio is between 10-20 molecules of biotin to protein. I can provide more specific information for a particular lot.


    I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

    Read More

    Abcam Scientific Support

    Answered on Feb 20 2012

    Question

    in relation to product ab8033 (biotinylated albumin), I have a further question for you: we would like to know what is the exact biotinylation group used to label ab8033. This is important to us as it will aid our MS scientist identify an ab8033-derived fragment that originated from our ongoing research project; it would be crucial for our work to know this detail. Below, I have pasted for you the relevant phrases our MS scientist used to describe this request. I will have to know exactly what biotinylation group you used - this modification would then be added to the database search as a variable modification to help locate the modified lysines. BUT, the calculation of the added biotin mass has to be exact to the hydrogen or I cannot locate the modified mass We will be looking forward to your response.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 07 2006

    Answer

    Thank you for your enquiry. The exact biotin group added was Biotinamidocaproate N-Hydroxysuccinimide Ester. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Feb 07 2006

    Question

    according to the Abcam datasheet, ab8033 appears to be not an antibody but a biotin-labeled albumin; i.e. Albumin protein (Biotin) (ab8033). In Purification Notes it states: "This product was prepared from chromatographically pure Human Albumin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Albumin.". But, in any case, if the information we got form your supplier is accurately referred to this product, it appears that the albumin molecule has been randomly labeled with biotin at Lys residues. Please confirm this last statement if possible/applicable.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 22 2005

    Answer

    Yes, you are correct. I am sorry if there was some confusion from my earlier response. ab8033 is not an antibody but rather biotin-labeled albumin. Yes, the molecule has been randomly labeled with biotin at Lys residues. Thank you for your enquiry and please do no hesitate to contact me if you have any further questions.

    Read More

    Abcam Scientific Support

    Answered on Apr 22 2005

    Question

    we would like to know where the biotin is attached in the albumin molecule; i.e. N-terminally, C-terminally, uniformally at Lys residues or else. Reason is we would like to use ab8033 as a substrate to examine putative specific proteolytic fragments on SDS-PAGE. Thanks in advance

    Read More

    Abcam community

    Verified customer

    Asked on Apr 18 2005

    Answer

    I have a response from our supplier and I want to thank you for your patience. The biotin is attached to the antibody via free primary amines, i.e. at Lys residues. If you have any further questions please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Apr 21 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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