Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
製品名Native human Plasmin protein
Plasmin タンパク質・ペプチド 製品一覧
生理活性Plasmin retains catalytic activity when coupled and may be used to efficiently activate single-chain tPA and uPA to the two-chain form
精製度> 95 % SDS-PAGE.
Human Lys plasmin immobilised on agarose resin by coupling of primary amines.
タンパク質長Full length protein
配列MEHKEVVLLLLLFLKSGQGEPLDDYVNTQGASLFSVTKKQLGAGSIEECA AKCEEDEEFT CRAFQYHSKEQQCVIMAENRKSSIIIRMRDVVLFEKKV YLSECKTGNGKNYRGTMSKTKN GITCQKWSSTSPHRPRFSPATHPSEG LEENYCRNPDNDPQGPWCYTTDPEKRYDYCDILE CEEECMHCSGENYD GKISKTMSGLECQAWDSQSPHAHGYIPSKFPNKNLKKNYCRNPDRE LR PWCFTTDPNKRWELCDIPRCTTPPPSSGPTYQCLKGTGENYRGNVAVTVS GHTCQHWS AQTPHTHNRTPENFPCKNLDENYCRNPDGKRAPWCHTTNS QVRWEYCKIPSCDSSPVSTE QLAPTAPPELTPVVQDCYHGDGQSYRGT SSTTTTGKKCQSWSSMTPHRHQKTPENYPNAG LTMNYCRNPDADKGPW CFTTDPSVRWEYCNLKKCSGTEASVVAPPPVVLLPDVETPSEED CMFG NGKGYRGKRATTVTGTPCQDWAAQEPHRHSIFTPETNPRAGLEKNYCRNP DGDVGG PWCYTTNPRKLYDYCDVPQCAAPSFDCGKPQVEPKKCPGRVV GGCVAHPHSWPWQVSLRT RFGMHFCGGTLISPEWVLTAAHCLEKSPRP SSYKVILGAHQEVNLEPHVQEIEVSRLFLE PTRKDIALLKLSSPAVIT DKVIPACLPSPNYVVADRTECFITGWGETQGTFGAGLLKEAQ LPVIEN KVCNRYEFLNGRVQSTELCAGHLAGGTDSCQGDSGGPLVCFEKDKYILQG VTSW GLGCARPNKPGVYVRVSRFVTWIEGVMRNN
Our Abpromise guarantee covers the use of ab95112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Shipped at 4°C. Store at +4°C.
Preservative: 0.02% Sodium azide
Constituents: 2.38% HEPES, 0.58% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Plasmin light chain B
機能Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.
Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo.
組織特異性Present in plasma and many other extracellular fluids. It is synthesized in the liver.
配列類似性Belongs to the peptidase S1 family. Plasminogen subfamily.
Contains 5 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain.
ドメインKringle domains mediate interaction with CSPG4.
翻訳後修飾N-linked glycan contains N-acetyllactosamine and sialic acid. O-linked glycans consist of Gal-GalNAc disaccharide modified with up to 2 sialic acid residues (microheterogeneity).
In the presence of the inhibitor, the activation involves only cleavage after Arg-580, yielding two chains held together by two disulfide bonds. In the absence of the inhibitor, the activation involves additionally the removal of the activation peptide.
細胞内局在Secreted. Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.