Application
Other - Do not use
Review text: The protein can be used reduced + denaturated (band size ~130 kDa)
or native (two bands ~ 130kDa and ~300kDa)
The native protein gives a brighter signal.
PH of the proteinlysate is low - a loading dye with bromphenolblue changes its colour. This does not effect the western blot.
Sample: Human Purified protein
Primary antibody (in addition to 'Collagen III protein')
Primary antibody: Abcam primary antibody: Anti-Collagen III antibody [FH-7A] (ab6310)
Dilution: 1/500
Secondary antibody
Name: Non-Abcam antibody was used: goat anti mouse IgG-HRP, Santa Cruz-2005, lot c100
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution: 1/2000
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Michaela Leyh
Verified customer
投稿 Feb 09 2011