Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Suitable for: Purification, SDS-PAGE
製品名Native Human Antithrombin III/ATIII protein
Antithrombin III/ATIII タンパク質・ペプチド 製品一覧
精製度> 95 % SDS-PAGE.
タンパク質長Full length protein
Our Abpromise guarantee covers the use of ab95077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
2ml of 1:1 slurry
1.0 mg/ml coupled
May be used repeatedly. Protocol for purifying high affinity heparin with immobilized antithrombin:
1. Equilibrate immobilized antithrombin in TBS (0.1M Tris-HCl, 0.15M NaCl, pH 7.4) or PBS (0.05M Sodium Phosphate, 0.15M NaCl, pH 7.4).
2. Apply heparin in TBS or PBS. Binding capacity is ~0.1 mg high affinity heparin / ml resin and must be determined by the end user.
3. Wash and elute heparin with 3M NaCl in TBS or PBS.
4. Re-equilabrate resin in TBS or PBS.
5. Add 0.02percent Sodium Azide for storage.
Previously labelled as Antithrombin III.
Concentration information loading...
Shipped at 4°C. Store at +4°C.
Preservative: 0.02% Sodium azide
Constituents: 1.58% Tris HCl, 0.87% Sodium chloride
- Antithrombin III
機能Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
組織特異性Found in plasma.
関連疾患Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations.
配列類似性Belongs to the serpin family.
翻訳後修飾Phosphorylation sites are present in the extracelllular medium.
細胞内局在Secreted > extracellular space.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.