Native human alpha 1 Antitrypsin protein (Active) (ab91136)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Achieve higher consistency and quality standards with a premium grade bioactive protein
- High batch-to-batch consistency
- Optimal bioactivity
- Guaranteed identical to human native proteins
- >95% purity
- Ultra-low endotoxin levels: <0.005 Eu/µg
- Carrier and tag free
製品の詳細
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製品名
Native human alpha 1 Antitrypsin protein (Active)
alpha 1 Antitrypsin タンパク質・ペプチド 製品一覧 -
生理活性
When tested with active-site titrated porcine pancreatic trypsin using Na-Benzoyl-L-Arginine-para-Nitroanilide Hydrochloride (L-BAPNA) as substrate, it is 75-100% inhibitory.
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精製度
> 95 % SDS-PAGE. -
発現系
Native -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Native -
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生物種
Human -
予測される分子量
52 kDa
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab91136 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
Functional Studies
SDS-PAGE
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製品の状態
Lyophilized -
備考
Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 0.433
Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at -80°C.
pH: 6.50
Constituents: 0.492% Sodium phosphate, 1.74% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with distilled water. Once reconstituted, ab91136 is stable for one week at 4°C.
関連情報
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別名
- A1A
- A1AT
- A1AT_HUMAN
see all -
機能
Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). -
組織特異性
Ubiquitous. Expressed in leukocytes and plasma. -
関連疾患
Alpha-1-antitrypsin deficiency -
配列類似性
Belongs to the serpin family. -
ドメイン
The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable. -
翻訳後修飾
N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418. -
細胞内局在
Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix. - Information by UniProt
画像
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SDS-PAGE: 4-12% Bis-Tris NuPAGE gel
Lane 1. 5 μg ab91136 (reduced/heated)
Lane 2. 10 μg ab91136 (reduced/heated)
Lane 3. 20 μg ab91136 (reduced/heated)
Lane 4. Molecular weight markers
Lane 5. 5 μg ab91136 (non-reduced/no heat)
Lane 6. 10 μg ab91136 (non-reduced/no heat)
Lane 7. 20 μg ab91136 (non-reduced/no heat) -
Anti-alpha 1 Antitrypsin antibody [B9] (ab9399) at 1 µg/ml +
Native human alpha 1 Antitrypsin protein (Active) (ab91136) at 0.1 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 30 seconds
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab91136 は 2 報の論文で使用されています。
- Esteghamat F et al. CELA2A mutations predispose to early-onset atherosclerosis and metabolic syndrome and affect plasma insulin and platelet activation. Nat Genet 51:1233-1243 (2019). PubMed: 31358993
- Muley MM et al. Prophylactic inhibition of neutrophil elastase prevents the development of chronic neuropathic pain in osteoarthritic mice. J Neuroinflammation 14:168 (2017). PubMed: 28835277