Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE
製品名Native Hamster Fibronectin protein
Fibronectin タンパク質・ペプチド 製品一覧
精製度> 95 % SDS-PAGE.
Purified from hamster plasma
タンパク質長Full length protein
Our Abpromise guarantee covers the use of ab92798 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
備考Thaw the Fibronectin by placing the vial in a 37°C water bath and leaving it undisturbed until completely thawed. Do not disturb the vial at any time during the thawing process. If the vial is disturbed or removed prior to complete thawing, the fibronectin will form a gel and be unusable. Mix very gently with pipette after thawing. Vortexing, excessive agitation, repeated freezing and thawing of Fibronectin are not recommended.
Concentration information loading...
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 1.58% Tris HCl, 0.87% Sodium chloride
- Cold insoluble globulin
- Cold-insoluble globulin
機能Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
組織特異性Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
関連疾患Glomerulopathy with fibronectin deposits 2
配列類似性Contains 12 fibronectin type-I domains.
Contains 2 fibronectin type-II domains.
Contains 16 fibronectin type-III domains.
発生段階Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
Phosphorylated by FAM20C in the extracellular medium.
Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
細胞内局在Secreted, extracellular space, extracellular matrix.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab92798 は 1 報の論文で使用されています。
- Lambshead JW et al. Defining synthetic surfaces for human pluripotent stem cell culture. Cell Regen (Lond) 2:7 (2013). PubMed: 25408879