Anti-NAT1 抗体 [EPR3221(2)] (ab109114)
![Western blot - Anti-NAT1 antibody [EPR3221(2)] (ab109114)](https://www.abcam.co.jp/ps/products/109/ab109114/Images/ab109114-121683-anti-nat1-antibody-epr32212-western-blot.jpg "Western blot - Anti-NAT1 antibody [EPR3221(2)] (ab109114)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3221(2)] to NAT1
- Suitable for: WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-NAT1 antibody [EPR3221(2)]
NAT1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3221(2)] to NAT1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, Flow Cyt (Intra)more details
適用なし: IHC-P or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- U937, fetal liver, LnCaP, human bladder, and HT-1376 cell lysates and LN-CAP cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3221(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109114の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 34 kDa.
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| ICC/IF |
1/100 - 1/250.
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| Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 34 kDa. |
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ICC/IF
1/100 - 1/250. |
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Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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ターゲット情報
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機能
Participates in the detoxification of a plethora of hydrazine and arylamine drugs. Catalyzes the N- or O-acetylation of various arylamine and heterocyclic amine substrates and is able to bioactivate several known carcinogens. -
配列類似性
Belongs to the arylamine N-acetyltransferase family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 9 Human
- Omim: 108345 Human
- SwissProt: P18440 Human
- Unigene: 2 Human
- Unigene: 591847 Human
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別名
- AAC1 antibody
- ARY1_HUMAN antibody
- Arylamide acetylase 1 antibody
see all
画像
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Western blot - Anti-NAT1 antibody [EPR3221(2)] (ab109114)All lanes : Anti-NAT1 antibody [EPR3221(2)] (ab109114) at 1/1000 dilution
Lane 1 : U937 cell lysate
Lane 2 : Fetal liver cell lysate
Lane 3 : LnCaP cell lysate
Lane 4 : Human bladder cell lysate
Lane 5 : HT-1376 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 34 kDa -
![Flow Cytometry (Intracellular) - Anti-NAT1 antibody [EPR3221(2)] (ab109114)](https://www.abcam.co.jp/ps/products/109/ab109114/Images/ab109114-4-anti-nat1-antibody-epr3221-2-flowcytometry-lncap-human.jpg)
Flow Cytometry (Intracellular) - Anti-NAT1 antibody [EPR3221(2)] (ab109114)Flow Cytometry analysis of LNCaP (Human prostate carcinoma epithelial cell) cells labeling NAT1 with purified ab109114 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Immunocytochemistry/ Immunofluorescence - Anti-NAT1 antibody [EPR3221(2)] (ab109114)](https://www.abcam.co.jp/ps/products/109/ab109114/Images/ab109114-121682-anti-nat1-antibody-epr32212-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NAT1 antibody [EPR3221(2)] (ab109114)Immunofluorescent staining of LN-CAP cells using anti-NAT1 at 1/100 (ab109114). -
![Anti-NAT1 antibody [EPR3221(2)] (ab109114)](https://www.abcam.co.jp/ps/products/109/ab109114/Images/ab109114-3-benefits-of-recombinant-antibodies.png)
Anti-NAT1 antibody [EPR3221(2)] (ab109114)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab109114 は 3 報の論文で使用されています。
- Salazar-González RA et al. Human arylamine N-acetyltransferase 2 genotype-dependent protein expression in cryopreserved human hepatocytes. Sci Rep 10:7566 (2020). PubMed: 32372066
- Stepp MW et al. N-Acetyltransferase 1 Knockout Elevates Acetyl Coenzyme A Levels and Reduces Anchorage-Independent Growth in Human Breast Cancer Cell Lines. J Oncol 2019:3860426 (2019). PubMed: 31531019
- Hernández-González O et al. Genetic polymorphisms of arylamine N-acetyltransferases 1 and 2 and the likelihood of developing pediatric acute lymphoblastic leukemia. Leuk Lymphoma 59:1968-1975 (2018). PubMed: 29214875