Anti-NAP1L4 抗体 (ab21631)
Key features and details
- Rabbit polyclonal to NAP1L4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-NAP1L4 antibody -
製品の詳細
Rabbit polyclonal to NAP1L4 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Dog
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human NAP1L4.
Immunogen の所有権に関して (Peptide available as ab22419.) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab21631の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/250 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 43 kDa).
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| 特記事項 |
|---|
|
WB
1/250 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 43 kDa). |
ターゲット情報
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関連性
This gene encodes a member of the nucleosome assembly protein (NAP) family which can interact with both core and linker histones. It can shuttle between the cytoplasm and nucleus, suggesting a role as a histone chaperone. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 4676 Human
- Omim: 601651 Human
- SwissProt: Q99733 Human
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別名
- NAP2 antibody
- Nucleosome assembly protein 1-like 4 antibody
- Nucleosome assembly protein 2 antibody
画像
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Western blot - Anti-NAP1L4 antibody (ab21631)Lanes 1-2 : Anti-NAP1L4 antibody (ab21631) at 1/1000 dilution
Lanes 3-4 : Anti-NAP1L4 antibody (ab21631) at 1/500 dilution
Lanes 5-6 : Anti-NAP1L4 antibody (ab21631) at 1/250 dilution
Lanes 1 & 3 & 5 : U2OS whole cell lysate
Lanes 2 & 4 & 6 : U2OS whole cell lysate, treated with ionizing radiation
Developed using the ECL technique.
Predicted band size: 43 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa (possible cleavage fragment), 30 kDa (possible cross reactivity), 64 kDa (possible cross reactivity)For each lane, U2OS cells, either treated or not treated with ionizing radiation, were scraped from a 60 mm dish and added to 75 µl of 2X Laemmli buffer. 20 µl of these samples were loaded into each lane. ab21631 recognizes a major band of approximately 52 kDa corresponding closely in size to NAP1L4, which has been shown to migrate at approximately 50-55 kDa (Rodriquez, et al., J. of Cell. Biochemistry 93:398-408). It also detects a cross-reacting band at 30 kDa.
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Western blot - Anti-NAP1L4 antibody (ab21631)All lanes : Anti-NAP1L4 antibody (ab21631) at 1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa (possible cleavage fragment), 30 kDa (possible cross reactivity)
ab21631 recognizes a major band of approximately 52 kDa corresponding closely in size to NAP1L4, which has been shown to migrate at approximately 50-55 kDa (Rodriquez, et al., J. of Cell. Biochemistry 93:398-408). It also detects a cross-reacting band at 30 kDa.
データシートおよび資料
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Datasheet download
参考文献 (3)
ab21631 は 3 報の論文で使用されています。
- Spiniello M et al. HyPR-MS for Multiplexed Discovery of MALAT1, NEAT1, and NORAD lncRNA Protein Interactomes. J Proteome Res 17:3022-3038 (2018). PubMed: 29972301
- Aslanian A et al. Mass spectrometry-based quantification of the cellular response to methyl methanesulfonate treatment in human cells. DNA Repair (Amst) 15:29-38 (2014). PubMed: 24461736
- Cho I et al. ATP-dependent chromatin remodeling by Cockayne syndrome protein B and NAP1-like histone chaperones is required for efficient transcription-coupled DNA repair. PLoS Genet 9:e1003407 (2013). IP ; Human . PubMed: 23637612
