Anti-Nanog 抗体 (ab80892)
Key features and details
- Rabbit polyclonal to Nanog
- Suitable for: ICC, ICC/IF, IHC-P, Flow Cyt, IHC - Wholemount, WB
- Reacts with: Mouse
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Nanog antibody
Nanog 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Nanog -
由来種
Rabbit -
アプリケーション
適用あり: ICC, ICC/IF, IHC-P, Flow Cyt, IHC - Wholemount, WBmore details -
種交差性
交差種: Mouse -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab80892の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC | (1) |
Use at an assay dependent concentration.
|
ICC/IF | (5) |
Use at an assay dependent concentration.
|
IHC-P | (5) |
Use at an assay dependent concentration.
|
Flow Cyt | (2) |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC - Wholemount | (1) |
Use at an assay dependent concentration.
|
WB | (3) |
Use at an assay dependent concentration.
|
特記事項 |
---|
ICC
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC - Wholemount
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
ターゲット情報
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機能
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation. -
組織特異性
Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. -
配列類似性
Belongs to the Nanog homeobox family.
Contains 1 homeobox DNA-binding domain. -
発生段階
Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 71950 Mouse
- SwissProt: Q80Z64 Mouse
- Unigene: 6047 Mouse
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別名
- Embryonic stem cell specific homeobox protein (Nanog) antibody
- ENK antibody
- FLJ12581 antibody
see all
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (335)
ab80892 は 335 報の論文で使用されています。
- Lee J et al. Production of large, defined genome modifications in rats by targeting rat embryonic stem cells. Stem Cell Reports 18:394-409 (2023). PubMed: 36525967
- Wakayama S et al. Healthy cloned offspring derived from freeze-dried somatic cells. Nat Commun 13:3666 (2022). PubMed: 35790715
- Xu M et al. Genome-scale screening in a rat haploid system identifies Thop1 as a modulator of pluripotency exit. Cell Prolif 55:e13209 (2022). PubMed: 35274380
- Yan S et al. CTGF Promotes the Osteoblast Differentiation of Human Periodontal Ligament Stem Cells by Positively Regulating BMP2/Smad Signal Transduction. Biomed Res Int 2022:2938015 (2022). PubMed: 36158888
- Song JH et al. Developmental Potency and Metabolic Traits of Extended Pluripotency Are Faithfully Transferred to Somatic Cells via Cell Fusion-Induced Reprogramming. Cells 11:N/A (2022). PubMed: 36291134