Application
Western blot
Sample
Mouse Cell lysate - whole cell (ES cell)
Loading amount
10 µg
Specification
ES cell
Treatment
2000 U/ml mouse LIF constantly
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Other product details
Dilution
1/2000
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/30000
Detection
Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: around 40 kDa Non-specific: 55 kDa
Additional data
Additional Notes
Lane 1: E14 cell line cultured with LIF.
Lane 2: E14 cell line cultured without LIF.
Lane 2: E14 cell line cultured without LIF.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Feb 12 2009