Anti-NAIP 抗体 [EPR6169] - BSA and Azide free (ab248285)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6169] to NAIP - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-NAIP antibody [EPR6169] - BSA and Azide free
NAIP 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6169] to NAIP - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- WB: Human tonsil and ?Human placenta tissue lysates.
-
特記事項
ab248285 is the carrier-free version of ab129079.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
解離定数(KD 値)
KD = 1.33 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6169 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248285の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use at an assay dependent concentration. Detects a band of approximately 160 kDa (predicted molecular weight: 160 kDa).
|
特記事項 |
---|
WB
Use at an assay dependent concentration. Detects a band of approximately 160 kDa (predicted molecular weight: 160 kDa). |
ターゲット情報
-
機能
Prevents motor-neuron apoptosis induced by a variety of signals. Possible role in the prevention of spinal muscular atrophy that seems to be caused by inappropriate persistence of motor-neuron apoptosis: mutated or deleted forms of NAIP have been found in individuals with severe spinal muscular atrophy. -
組織特異性
Expressed in motor neurons, but not in sensory neurons. Found in liver and placenta, and to a lesser extent in spinal cord. -
配列類似性
Contains 3 BIR repeats.
Contains 1 NACHT domain. - Information by UniProt
-
参照データベース
- Entrez Gene: 100510692 Human
- Entrez Gene: 4671 Human
- Omim: 600355 Human
- SwissProt: Q13075 Human
- Unigene: 654500 Human
- Unigene: 710305 Human
-
別名
- Baculoviral IAP repeat containing 1 antibody
- Baculoviral IAP repeat-containing protein 1 antibody
- BIRC 1 antibody
see all
画像
-
All lanes : Anti-NAIP antibody [EPR6169] (ab129079) at 1/20000 dilution (Purified)
Lane 1 : Human placenta lysate
Lane 2 : Human tonsil lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 160 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?This data was developed using ab129079, the same antibody clone in a different buffer formulation. We are unsure about the nature of the extra bands.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
Certificate of Compliance
参考文献 (0)
ab248285 は論文での使用が確認できていません。