The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 160 kDa (predicted molecular weight: 160 kDa).Can be blocked with Human NAIP peptide (ab39795).
Use at an assay dependent concentration. PubMed: 20844241
Use at an assay dependent concentration.
Use a concentration of 10 µg/ml.
Prevents motor-neuron apoptosis induced by a variety of signals. Possible role in the prevention of spinal muscular atrophy that seems to be caused by inappropriate persistence of motor-neuron apoptosis: mutated or deleted forms of NAIP have been found in individuals with severe spinal muscular atrophy.
Expressed in motor neurons, but not in sensory neurons. Found in liver and placenta, and to a lesser extent in spinal cord.
IHC image of ab25968 staining in human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25968, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-NAIP antibody (ab25968)This image is courtesy of an Abreview submitted by Dr Mahesh Shivananjappa
Anti-NAIP antibody (ab25968) at 1/1500 dilution + Mouse RAW 264.7 cell lysate at 20 µg
Secondary HRP Goat anti-rabbit IgG polyclonal at 1/10000 dilution