Anti-NAIP 抗体 (ab2549)

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Key features and details

  • Rabbit polyclonal to NAIP
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-NAIP antibody
    NAIP 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to NAIP

  • 由来種

    Rabbit

  • 特異性

    This antibody detects recombinant human NAIP in extracts from Sf21 insect cells, but it is unknown as to whether this antibody detects the endogenous form.

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide surrounding amino acid 506 of human NAIP.

  • 特記事項


    NAIP (Neuronal Apoptosis Inhibitor Protein) is a member of the IAPs (Inhibitor of Apoptosis Proteins) that function in cell death pathways to inhibit apoptosis. NAIP was discovered based on its association with a neurodegenerative disorder. Mutations and deletions in the NAIP gene locus is a contributing factor in spinal muscular atrophy. Coexpression of NAIP and hippocalcin protects neurons against calcium-induced cell death. NAIP is strongly expressed in anterior horn and motor cortex neurons of the normal brain. It is also found in human fetal neurons and in adult choroid plexus cells.

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製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • バッファー

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 1% BSA
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 一次抗体 備考

    NAIP (Neuronal Apoptosis Inhibitor Protein) is a member of the IAPs (Inhibitor of Apoptosis Proteins) that function in cell death pathways to inhibit apoptosis. NAIP was discovered based on its association with a neurodegenerative disorder. Mutations and deletions in the NAIP gene locus is a contributing factor in spinal muscular atrophy. Coexpression of NAIP and hippocalcin protects neurons against calcium-induced cell death. NAIP is strongly expressed in anterior horn and motor cortex neurons of the normal brain. It is also found in human fetal neurons and in adult choroid plexus cells.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 140 kDa.

ターゲット情報

  • 機能

    Prevents motor-neuron apoptosis induced by a variety of signals. Possible role in the prevention of spinal muscular atrophy that seems to be caused by inappropriate persistence of motor-neuron apoptosis: mutated or deleted forms of NAIP have been found in individuals with severe spinal muscular atrophy.

  • 組織特異性

    Expressed in motor neurons, but not in sensory neurons. Found in liver and placenta, and to a lesser extent in spinal cord.

  • 配列類似性

    Contains 3 BIR repeats.
    Contains 1 NACHT domain.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Baculoviral IAP repeat containing 1 antibody
      • Baculoviral IAP repeat-containing protein 1 antibody
      • BIRC 1 antibody
      • BIRC1 antibody
      • BIRC1_HUMAN antibody
      • Birc1a antibody
      • FLJ42520 antibody
      • NAIP antibody
      • Naip1 antibody
      • Neuronal apoptosis inhibitory protein antibody
      • NLR family apoptosis inhibitory protein antibody
      • NLR family BIR domain containing 1 antibody
      • NLRB 1 antibody
      • NLRB1 antibody
      • Nucleotide binding oligomerization domain leucine rich repeat and BIR domain containing 1 antibody
      • Psi neuronal apoptosis inhibitory protein antibody
      • psiNAIP antibody
      • Similar to occludin antibody
      see all

    画像

    • Western blot - Anti-NAIP antibody (ab2549)
      Western blot - Anti-NAIP antibody (ab2549)Image from Dr MT Romanish et al, PLoS One. 2009 Jun 2;4(6):e5761, Fig 5.
      All lanes : Anti-NAIP antibody (ab2549) at 1/1000 dilution

      Lane 1 : Lysate prepared from transfected PC3 cells.
      Lane 2 : Lysate prepared from non-transfected PC3 cells.
      Lane 3 : Lysate prepared from transfected HeLa cells.
      Lane 4 : Lysate prepared from non-transfected HeLa cells.
      Lane 5 : Lysate prepared from non-transfected NTera2D1 cells.

      Secondary
      All lanes : secondary antibody at 1/100000 dilution

      Observed band size: 160 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 105 kDa (possible isoform), 115 kDa, 130 kDa (possible isoform). We are unsure as to the identity of these extra bands.



      Detection of novel NAIP protein isoforms.

      Cells were grown in 10 cm dishes. The human PC3, NTera2D1, and HeLa cell lines were selected to screen for NAIP proteins based on preliminary RT-PCR findings. Cells transfected with the expression vector encoding the Alu-derived NAIP ORF or untransfected controls were harvested by either scraping or trypsinization following two washes with cold PBS. Cell pellets were obtained by centrifugation and resuspended in RIPA (150 mM NaCl; 1% NP-40; 0.5% sodium deoxycholate; 0.1% SDS; 50 mM Tris, pH8) and NP40 (150 mM NaCl; 1% NP-40; 50 mM Tris, pH8) lysis buffers supplemented with a protease inhibitor cocktail, and subsequently quantified using the Qubit Fluorometer. RIPA provided clearer results for the NAIP-specific antibody. Bi-phased gels containing TEMED and APS (4% stacking, 9% separating) were used to resolve total cellular protein in electrophoresis running buffer (10×: 25 mM Tris; 192 mM glycine; 0.1% SDS). Subsequently, separa

    参考文献 (1)

    ab2549 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2549 は 1 報の論文で使用されています。

    • Romanish MT  et al. A novel protein isoform of the multicopy human NAIP gene derives from intragenic Alu SINE promoters. PLoS One 4:e5761 (2009). WB ; Human . PubMed: 19488400

    レビューと Q&A

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    レビューを送る 質問を送る

    Question

    Customer would like to know the sequence of the immunogen.

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    Answer

    a.a. 473-490 of human NAIP.

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