Anti-N6-methyladenosine (m6A) 抗体 [mAbcam 190886] - BSA and Azide free (ab190886)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [mAbcam 190886] to N6-methyladenosine (m6A) - BSA and Azide free
- Suitable for: Nucleotide Array
- Reacts with: Species independent
製品の概要
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製品名
Anti-N6-methyladenosine (m6A) antibody [mAbcam 190886] - BSA and Azide free
N6-methyladenosine (m6A) 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [mAbcam 190886] to N6-methyladenosine (m6A) - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: Nucleotide Arraymore details -
種交差性
交差種: Species independent -
免疫原
Chemical/ Small Molecule corresponding to N6-methyladenosine (m6A) conjugated to keyhole limpet haemocyanin.
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ポジティブ・コントロール
- ab190886 was tested on a oligonucleotide array against RNA oligomers; N6-methyladenosine (m6A) and unmodified adenosine. 2Ome-RNA oligomers carry a methyl group at the 2’-OH residue of the ribose molecule, making them resistant to most ribonucleases.
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特記事項
N6-methyladenosine (m6A) is a post-transcriptional modification of RNA. m6A modification has been identified in all classes of RNA (rRNA, tRNA and mRNA) and is catalysed by an evolutionary conserved multi-subunit enzyme, methyltransferase like 3 (METTL3). Cellular and viral RNA has been known to be methylated for decades. Recent studies have found that mRNA is predominately m6A modified at stop codons and long internal exons, which are conserved between mouse and human. Emerging evidence suggests m6A plays an important role in regulating gene expression, alternative splicing patterns, downstream signalling (p53) as well as apoptosis.
The regulation of m6A modifications in mRNA has been linked to disease, where fat mass and obesity-associated (FTO) has been has been reported to be a obesity risk gene. FTO is a m6A demethylase and polymorphisms that result in increased FTO expression are associated with increased body mass and risk of obesity.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Constituent: PBS
PBS prepared using DEPC-treated water.
The use of RNase inhibitor is recommended. -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
mAbcam 190886 -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab190886の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Nucleotide Array |
Use a concentration of 2 µg/ml.
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特記事項 |
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Nucleotide Array
Use a concentration of 2 µg/ml. |
ターゲット情報
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関連性
N6-Methyladenosine (m6A) is an abundant modification found in mRNA, tRNA, snRNA, as well as long non-coding RNA, in all species. RNA adenosine methylation is catalyzed by a multicomponent complex composed of METTL3/MT-A70, METTL14, and WTAP in mammals. METTL3 & METTL14 are responsible for the methyltransferase activity of the complex, and WTAP mediates substrate recruitment. -
別名
- m6A antibody
- N6-methyladenosine antibody
画像
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All batches of ab190886 are tested in Nucleotide Array against N6-methyladenosine and unmodified adenosine. Six dilutions of each oligomer are printed on to the Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to N6-methyladenosine, indicating that this antibody specifically recognises adenosine methylated at position N6.
2Ome-m6A - N6-methyladenosine
2Ome-m6A_unmod - unmodified adenosine
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (13)
ab190886 は 13 報の論文で使用されています。
- Wang L et al. METTL3 is a key regulator of milk synthesis in mammary epithelial cells. Cell Biol Int 46:359-369 (2022). PubMed: 34865263
- Shen D et al. METTL14-mediated Lnc-LSG1 m6A modification inhibits clear cell renal cell carcinoma metastasis via regulating ESRP2 ubiquitination. Mol Ther Nucleic Acids 27:547-561 (2022). PubMed: 35036065
- Wang Y et al. METTL14 promotes prostate tumorigenesis by inhibiting THBS1 via an m6A-YTHDF2-dependent mechanism. Cell Death Discov 8:143 (2022). PubMed: 35354789
- Xu B et al. Mettl3-mediated m6 A modification of Lrp2 facilitates neurogenesis through Ythdc2 and elicits antidepressant-like effects. FASEB J 36:e22392 (2022). PubMed: 35716070
- Duda KJ et al. m6A RNA methylation of major satellite repeat transcripts facilitates chromatin association and RNA:DNA hybrid formation in mouse heterochromatin. Nucleic Acids Res 49:5568-5587 (2021). PubMed: 33999208