Anti-N WASP (phospho Y256) 抗体 (ab23395)
Key features and details
- Rabbit polyclonal to N WASP (phospho Y256)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-N WASP (phospho Y256) antibody
N WASP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to N WASP (phospho Y256) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human N WASP (phospho Y256).
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ポジティブ･コントロール
- Pervanadate treated A431, HeLa, Jurkat and endothelial cells for SDS PAGE immunoblots.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This antibody was cross absorbed to phosphotyrosine then affinity purified using phospho N WASP (Tyr 256) peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab23395の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 55 kDa). The antibody detects a 63 or 65 kDa protein corresponding to phosphorylated N WASP on SDS PAGE immunoblots of A431, HeLa, Jurkat and endothelial cells treated with pervanadate. Weak bands are also observed at higher molecular weights after pervanadte treatment which may be due to low crossreactivity with phosphotyrosine.
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| 特記事項 |
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WB
1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 55 kDa). The antibody detects a 63 or 65 kDa protein corresponding to phosphorylated N WASP on SDS PAGE immunoblots of A431, HeLa, Jurkat and endothelial cells treated with pervanadate. Weak bands are also observed at higher molecular weights after pervanadte treatment which may be due to low crossreactivity with phosphotyrosine. |
ターゲット情報
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機能
Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. -
配列類似性
Contains 1 CRIB domain.
Contains 1 WH1 domain.
Contains 2 WH2 domains. -
細胞内局在
Cytoplasm > cytoskeleton. Nucleus. Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated. - Information by UniProt
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参照データベース
- Entrez Gene: 8976 Human
- Omim: 605056 Human
- SwissProt: O00401 Human
- Unigene: 143728 Human
- Unigene: 83916 Human
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別名
- DKFZp779G0847 antibody
- MGC48327 antibody
- N-WASP antibody
see all
画像
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Western blot - Anti-N WASP (phospho Y256) antibody (ab23395)All lanes : Anti-N WASP (phospho Y256) antibody (ab23395) at 1/10000 dilution
Lane 1 : Control A431 cells 20ug/lane
Lane 2 : Pervanadate-treated A431 cells 20 ug/lane
Predicted band size: 55 kDa
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
データシートおよび資料
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Datasheet download
参考文献 (6)
ab23395 は 6 報の論文で使用されています。
- Du G et al. Targeting Src family kinase member Fyn by Saracatinib attenuated liver fibrosis in vitro and in vivo. Cell Death Dis 11:118 (2020). PubMed: 32051399
- Zhang W et al. Rho kinase collaborates with p21-activated kinase to regulate actin polymerization and contraction in airway smooth muscle. J Physiol 596:3617-3635 (2018). PubMed: 29746010
- Zhang W et al. p21-Activated kinase (Pak) regulates airway smooth muscle contraction by regulating paxillin complexes that mediate actin polymerization. J Physiol 594:4879-900 (2016). PubMed: 27038336
- Rajput C et al. Neural Wiskott-Aldrich syndrome protein (N-WASP)-mediated p120-catenin interaction with Arp2-Actin complex stabilizes endothelial adherens junctions. J Biol Chem 288:4241-50 (2013). WB ; Human . PubMed: 23212915
- Chacón MR et al. Focal adhesion kinase regulates actin nucleation and neuronal filopodia formation during axonal growth. Development 139:3200-10 (2012). WB . PubMed: 22872087
- Zhang W et al. The small GTPase RhoA regulates the contraction of smooth muscle tissues by catalyzing the assembly of cytoskeletal signaling complexes at membrane adhesion sites. J Biol Chem 287:33996-4008 (2012). WB ; Dog . PubMed: 22893699
