Application
Western blot
Sample
Mouse Tissue lysate - whole (Kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Kidney
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Concentration
1.75 µg/ml
Secondary antibody
Dilution
1/3000
Name
Non-Abcam antibody was used: anti-Rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Negative control
none
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 48 kDa Non-specific: 28, 33 kDa
Positive control
my sample
Additional data
Additional Notes
Kidney total protein was prepared from 1-wk-old C57BL/6 male mice (Charles River)
lane 1-3: 30ug of total kidney protein extracted from 3 different 1-wk-old mice.
Remarks: apart from the band close to the predicted size (~48kDa), we consistently see 2 additional bands, a strong one at ~28kDa, and a weak band at ~33kDa.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Dec 30 2010