Anti-n-Myc/MYCN 抗体 (ab24193)
Key features and details
- Rabbit polyclonal to n-Myc/MYCN
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-n-Myc/MYCN antibody
n-Myc/MYCN 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to n-Myc/MYCN -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Chicken, Non human primates
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免疫原
Synthetic peptide corresponding to Human n-Myc/MYCN aa 1-100 conjugated to keyhole limpet haemocyanin. Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human n-Myc/MYCN.
Immunogen の所有権に関して (Peptide available as ab31595.)
Database link: P04198
(Peptide available asab31595, ab31596) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab24193の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (4) |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
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| 特記事項 |
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WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). |
ターゲット情報
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機能
May function as a transcription factor. -
関連疾患
Note=Amplification of the N-MYC gene is associated with a variety of human tumors, most frequently neuroblastoma, where the level of amplification appears to increase as the tumor progresses.
Defects in MYCN are the cause of microcephaly-oculo-digito-esophageal-duodenal syndrome (MODED) [MIM:164280]; also known as oculodigitoesophagoduodenal syndrome (ODED). Microcephaly-oculo-digito-esophageal-duodenal syndrome is characterized by variable combinations of esophageal and duodenal atresias, microcephaly, learning disability and limb malformations. Cardiac and renal malformations, vertebral anomalies, and deafness have also been described.
Defects in MYCN are the cause of microcephaly and digital abnormalities with normal intelligence (MCPHDANI) [MIM:602585]. -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
発生段階
Expressed during fetal development. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 4613 Human
- Entrez Gene: 18109 Mouse
- Entrez Gene: 298894 Rat
- Omim: 164840 Human
- SwissProt: P18444 Chicken
- SwissProt: P04198 Human
- SwissProt: P03966 Mouse
- SwissProt: Q63379 Rat
see all -
別名
- bHLHe37 antibody
- Class E basic helix-loop-helix protein 37 antibody
- MODED antibody
see all
画像
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Western blot - Anti-n-Myc/MYCN antibody (ab24193)All lanes : Anti-n-Myc/MYCN antibody (ab24193) at 1 µg/ml
Lane 1 : Heart (Human) Whole Cell Lysate - fetal normal tissue
Lane 2 : Heart (Mouse) Tissue Lysate
Lane 3 : Skeletal Muscle (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 49 kDa
Additional bands at: 190 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab24193 overnight at 4°C. Antibody binding was detected using an anti-rabbit IgG VHH single domain antibody conjugated to HRP (ab191866), and visualised using ECL development solution ab133406.
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Western blot - Anti-n-Myc/MYCN antibody (ab24193)All lanes : Anti-n-Myc/MYCN antibody (ab24193) at 1 µg/ml
Lane 1 : Heart (Human) Whole Cell Lysate - fetal normal tissue (ab29432)
Lane 2 : Heart (Human) Nuclear Lysate - fetal normal tissue (ab29428)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 49 kDa
Additional bands at: 25 kDa (possible cleavage fragment), 25 kDa (possible cross reactivity), 33 kDa (possible cleavage fragment), 33 kDa (possible cross reactivity), 39 kDa (possible cleavage fragment), 39 kDa (possible cross reactivity)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab24193 は 12 報の論文で使用されています。
- Wang XH et al. FGF represses metastasis of neuroblastoma regulated by MYCN and TGF-ß1 induced LMO1 via control of let-7 expression. Brain Res 1704:219-228 (2019). PubMed: 30321496
- Zhu S et al. BMP4 and Neuregulin regulate the direction of mouse neural crest cell differentiation. Exp Ther Med 17:3883-3890 (2019). PubMed: 31007733
- Deng J et al. ß2-adrenergic receptor signaling promotes neuroblastoma cell proliferation by activating autophagy. Oncol Rep N/A:N/A (2019). PubMed: 31524241
- Zhang L et al. Single-Cell Transcriptomics in Medulloblastoma Reveals Tumor-Initiating Progenitors and Oncogenic Cascades during Tumorigenesis and Relapse. Cancer Cell 36:302-318.e7 (2019). PubMed: 31474569
- Wang Y et al. Downregulation of N-Myc inhibits neuroblastoma cell growth via the Wnt/ß-catenin signaling pathway. Mol Med Rep 18:377-384 (2018). PubMed: 29749516