Anti-N epsilon gamma glutamyl Lysine 抗体 [81D4] (ab424)

Abreviews (2)Q&A (16)Specific References (12)

製品の概要

  • 製品名
    Anti-N epsilon gamma glutamyl Lysine antibody [81D4]
  • 製品の詳細
    Mouse monoclonal [81D4] to N epsilon gamma glutamyl Lysine
  • 由来種
    Mouse
  • アプリケーション
    適用あり: IHC-P, ELISA, IHC-Frmore details
  • 免疫原

    Synthetic isopeptide.

  • 特記事項
    The ability of this antibody to fix complement is unknown. The isopeptide may be purchased from Bachem Catalog number G-1970.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/800.
ELISA Use at an assay dependent concentration.
IHC-Fr 1/50 - 1/800.

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N epsilon gamma glutamyl Lysine antibody [81D4] (ab424)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N epsilon gamma glutamyl Lysine antibody [81D4] (ab424)
    ab424 staining N epsilon gamma glutamyl Lysine in skin tissue sections.
  • Immunohistochemistry (Frozen sections) - Anti-N epsilon gamma glutamyl Lysine antibody [81D4] (ab424)
    Immunohistochemistry (Frozen sections) - Anti-N epsilon gamma glutamyl Lysine antibody [81D4] (ab424)
    ab424 staining N epsilon gamma glutamyl Lysine in kidney tissue sections by Immunohistochemistry (Frozen sections). Sections A through D were stained for insoluble tissue transglutaminase, while E through H were stained for N epsilon gamma glutamyl Lysine crosslink. A, B, E, F were control kidneys; C, D, G, H were diseased kidneys showing increased staining in the expanded interstitium, the mesangium and periglomerular.

プロトコール

参考文献

This product has been referenced in:

See all 12 Publications for this product

ab424 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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18 reviews or Q&A

10
Votes: Highest First

Immunocytochemistry/ Immunofluorescence abreview for Anti-N epsilon gamma glutamyl Lysine antibody [81D4]

 Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Trabecular Meshwork)
Permeabilization
No
Specification
Trabecular Meshwork
Blocking step
PBS superblock blocking buffer (Thermo Scientific, Prod # 37517) as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

投稿 Jul 21 2015

It seems the IgM secondary antibody gave a good signal in at least some samples but only with overnight/4C incubation and not 2hr/RT. I run out of ab424 and I need to re-order. Can you check for me if the current lot you sell is still the same. I do no...

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Thank you very much for keeping me updated.

I'm glad to hear that the results seem to have improved. We currently have only that lot in stock (2 vials in our US stock and 1 vial in our UK stock), so there should be consistency in results. Read More

That would be great. I would order ab97230. You can ship at the address in my signature block below. Thank you, I appreciate it.

PS Would you mind sending along the Cell Imaging book (I have promo CELL-TBVCQ that expires Nov 16). Thank you!

Thank you very much for your reply.

I'm sending a free of charge vial of ab97230 on the order ***, which should ship out tonight and arrive tomorrow.

I've also included the promo code for the cell imaging book.

Please keep...

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If you have the same lot, there is no point to try it I guess. We are going
to try IgM. I found ab97230 and ab98679. Which one do you recommend?

Thank you very much for your reply.

For your assay, either of these antibodies will be suitable. I would suggest ab97230 because it is in stock and we can ship it to you overnight. Ab98679 is back-ordered and I expect it to work similarly to ...

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we did try also without antigen retrieval but we did not see any staining. I am currently using lot ***. I appreciate your offer of sending new ab. Would it be possible for me to ship you couple of slides, the same that we stained with no success,...

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Thank you very much for your reply.

I apologize but we won't be able to test the antibody with your samples in our lab at this time. I'm very sorry for the inconvenience. Did you check whether the mouse-on-mouse HRP polymer detection kit work...

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I contacted you a few weeks ago in regard to crosslinks antibody (ab424) that we used on formalin-fixed paraffin embedded aortic sections. We run a few tests changing the retrevial time, temperature of retrivial and reagent (citrate vs trypsin); we tri...

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Thank you very much for keeping me updated about the results.

I'm sorry to hear that altering the protocol did not help improve the results. Have you also tried staining sections without doing antigen retrieval at all? I apologize that we don...

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I gathered a few more info on the methods used with ab424:

1: antigen retrievial was done using a decloaking machine with Rodent Decloaking Agent. It was turned on 90C for 35 min with a cooldown of 85C for 10 min

2: blocking done ...

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Thank youvery much for your reply and for sending these additional details.

I have a couple of suggestions that might help improve the results. It may be useful to decrease the antigen retrieval time to 15-20 minutes, in order to reduce the r...

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Ifinally got your e-mail. Thanks for looking into this issue. We did antigen retrieval with an unmasking reagent in decloaking chamber at 90C. I know that this cross-links once formed are very stable so even rush conditions should not break them howeve...

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Thank you very much for your reply, and for your patience.

Thank you also for sending the images of your staining results. I've reviewed some of the literature in which this antibody was used in IHC-P, and there aren't many more details about...

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Was antigen retrieval used on formalin-fixed paraffin-embedded tissue?
This is a crosslinked epitope and antigen retrieval reverses crosslinks, so it may be an unsuitable step.

Thank you very much for your call last week and for your patience while I've looked into your enquiry.

We have not tested this antibody in our own labs in IHC on formalin-fixed paraffin-embedded tissue, but it has been testedin IHC-P accordin...

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May I check with you, for the N epsilon gamma glutamyl Lysine antibody [153-81D4] (ab424), the species reactivity is not stated in the antibody datasheet, so could I just confirm with you that I will be able to use this antibody for human cells?

I can confirm that this antibody will detect N epsilon gamma glutamyl Lysine also if part of a human protein. In the publication PubMed ID 18235007 for example, researchers have used this antibody successfully in human paraffin embedded tissue slides. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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