Anti-N-epsilon acetyl Lysine 抗体 [11A1] (ab409)

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Western blot - Anti-N-epsilon acetyl Lysine antibody [11A1] (ab409)

    Key features and details

    • Mouse monoclonal [11A1] to N-epsilon acetyl Lysine
    • Suitable for: WB, ELISA, IHC-P
    • Reacts with: Species independent
    • Isotype: IgG1

    製品の概要

    • 製品名

      Anti-N-epsilon acetyl Lysine antibody [11A1]

    • 製品の詳細

      Mouse monoclonal [11A1] to N-epsilon acetyl Lysine

    • 由来種

      Mouse

    • アプリケーション

      適用あり: WB, ELISA, IHC-Pmore details

    • 種交差性

      交差種: Species independent

    • 特記事項

      The ability of this antibody to fix complement is unknown.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab409の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    1/500 - 1/5000.
    ELISA
    1/5000 - 1/50000.
    IHC-P
    1/500 - 1/5000.
    特記事項
    WB
    1/500 - 1/5000.
    ELISA
    1/5000 - 1/50000.
    IHC-P
    1/500 - 1/5000.

    画像

    • Western blot - Anti-N-epsilon acetyl Lysine antibody [11A1] (ab409)
      Western blot - Anti-N-epsilon acetyl Lysine antibody [11A1] (ab409)Image from Pehar M et al., J Biol Chem. 2012 Jun 29;287(27):22436-40. Epub 2012 May 24.; Fig 2.; doi: 10.1074/jbc.C112.362871; June 29, 2012, The Journal of Biological Chemistry, 287, 22436-22440.
      Western Blot analysis showing ab409 staining N-epsilon acetyl Lysine on immunoprecipitated ER-resident proteins from control (non-transfected) H4 cells.

    参考文献 (8)

    ab409 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab409 は 8 報の論文で使用されています。

    • Peng Y  et al. Deficient import of acetyl-CoA into the ER lumen causes neurodegeneration and propensity to infections, inflammation, and cancer. J Neurosci 34:6772-89 (2014). PubMed: 24828632
    • Ding Y  et al. Biochemical Inhibition of the Acetyltransferases ATase1 and ATase2 Reduces ß-Secretase (BACE1) Levels and Aß Generation. J Biol Chem 287:8424-33 (2012). WB . PubMed: 22267734
    • Pehar M  et al. Proteomic assessment shows that many ER-resident proteins are targeted by N{varepsilon}-lysine acetylation in the lumen of the organelle and predicts broad biological impact. J Biol Chem : (2012). WB . PubMed: 22628546
    • Guo B  et al. Dynamic modification of the ETS transcription factor PEA3 by sumoylation and p300-mediated acetylation. Nucleic Acids Res 39:6403-13 (2011). WB . PubMed: 21543453
    • Choi JK  et al. Acetylation of Rsc4p by Gcn5p is essential in the absence of histone H3 acetylation. Mol Cell Biol 28:6967-72 (2008). WB . PubMed: 18809572
    • Costantini C  et al. A reversible form of lysine acetylation in the ER and Golgi lumen controls the molecular stabilization of BACE1. Biochem J 407:383-95 (2007). WB . PubMed: 17425515
    • Mantovani F  et al. The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP. Nat Struct Mol Biol 14:912-20 (2007). PubMed: 17906639
    • Mantovani F  et al. Pin1 links the activities of c-Abl and p300 in regulating p73 function. Mol Cell 14:625-36 (2004). PubMed: 15175157

    レビューと Q&A

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    1-10 of 11 Abreviews or Q&A

    Question

    Could you please tell me what CBZ is and is the CBZ acetylated or is it the KLH? Thank you.

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    Answer

    The carboxybenzyloxy (CBZ) is a blocking group used to protect the primary amine during organic synthesis. We have used this derivative for the immunogen synthesis. There was no acetylated group on the immunogen.

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    Question

    Was the antigen that was used?

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    Answer

    The immunogen used to prepare this mouse monoclonal antibody was the KLH-lysine Nalpha-CBZ If you have any additional questions, please contact us again.

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    Question

    Dear Jennifer, the antibody lot number is 24382 the order number is XA0WSU the antibody arrived fully hydrated rather than lyophilized. i think that may have been the problem. Sincerely, Andy

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    Answer

    Ab409 used to be sold in lyophilized form and is now sold as a liquid. I apologize for the mistake on the datasheet and it has since been updated. Would you like a replacement vial of ab409? Please let me know and I can have it sent to you next week.

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    Question

    One of our clients would like to know whether the peptides you tested included sequences from the p53 and/or p65 (from NFkB) proteins. If yes, did Abcam's Ab recognize them? If not, did the other Ab you use recognize them?

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    Answer

    The suppier of ab409 was able to inform us that Ab409 was not tested on p53 and p65.

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    Question

    Hi: I am a graduate student at University of New Mexico. I bought the anti-acetyl Lysine (ab409) and anti-arginine (ab412) antibody for Western blot from you. I am just wondering that what kind of blotting reagent I should use for these two antibody: skim milk or BSA in TBST. Thanks for your help.

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    Answer

    We would suggest using BSA in TBST solution. Do not hesite to contact us for any further information.

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    Question

    I have Anti-acetyl-histone polyclonal from your company. The catalog no is NB 500-108.I wish to use it for western blots of acetylated histones & also to detect any other acetylated protein.My question is 1.Is this the appropriate antibody for this purpose? 2.What is the dilution that I should use?

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    Answer

    Yes, ab409 -- Mouse monoclonal (11A1) to N-epsilon acetyl lysine is suitable for use in detecting acetylated histones and other acetylated proteins. Suggested dilution for Western blot is 1/500 to 1/5000.

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    Question

    Do you know which acetylated histones are recognised by ab409?

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    Answer

    This antibody should recognise all acetylated histones.

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    Question

    I'm looking for an antibody that recognizes acetylated lysines on proteins and that is not specific for histones. Could you please tell me what antibody (the ab409 or the ab193?) is the most suitable for westerns and supershifts experiments? thank you very much

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    Answer

    Both antibodies have been found to work well in WB. However, neither have been assessd in a supershift experiment, therefore we can't guarantee results.

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    Question

    dear sir/madam please let me know whether you have an anti-acetyl-lysine antibody suitable for emsa studies on non-histone acetylated proteins. thanks in advance nick

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    Answer

    All the information we have for this antibody is on the datasheet and at present, this is the only antibody agaisnt acetyl-lysine that we currently have in our product range.

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    Question

    please inform me if it is possible to purchase a trial size of this antibody

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    Answer

    Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we offer a replacement or reimbursement. We do not routinely sell samples. We also offer a USD20 Amazon gift voucher to researchers who feedback information about our products.

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    1-10 of 11 Abreviews or Q&A

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