Anti-MYRIP 抗体 (ab10149)
Key features and details
- Goat polyclonal to MYRIP
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MYRIP antibody
MYRIP 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to MYRIP -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide: KDLMEPALESAVMY, corresponding to C terminal amino acids 846-859 of Human MYRIP.
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特記事項
A molecular complex composed of Rab27A, MYRIP and myosin VIIa bridges retinal melanosomes to the actin cytoskeleton and thereby mediates the local trafficking of these organelles. The defect of this molecular complex is likely to account for the perinuclear mislocalization of the melanosomes observed in the retinal pigment epithelium cells of myosin VIIa-defective mice.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
一次抗体 備考
A molecular complex composed of Rab27A, MYRIP and myosin VIIa bridges retinal melanosomes to the actin cytoskeleton and thereby mediates the local trafficking of these organelles. The defect of this molecular complex is likely to account for the perinuclear mislocalization of the melanosomes observed in the retinal pigment epithelium cells of myosin VIIa-defective mice. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10149の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 96 kDa.
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
WB
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 96 kDa. 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor proteins MYO5A and MYO7A. May link RAB27A-containing vesicles to actin filaments. -
組織特異性
Detected in brain, skin, heart, adrenal medulla, pancreas, intestine, liver, kidney, muscle and testis. -
配列類似性
Contains 1 FYVE-type zinc finger.
Contains 1 RabBD (Rab-binding) domain. -
細胞内局在
Cytoplasm. In pre- and post-synaptic areas in photoreceptor cells and in the basal microvilli of retinal pigment epithelium cells. Associated with melanosomes. Colocalizes with actin filaments. - Information by UniProt
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参照データベース
- Entrez Gene: 25924 Human
- Omim: 611790 Human
- SwissProt: Q8NFW9 Human
- Unigene: 594535 Human
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別名
- DKFZp586F1018 antibody
- Exophilin 8 antibody
- Exophilin antibody
see all
画像
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Western blot - Anti-MYRIP antibody (ab10149)Anti-MYRIP antibody (ab10149) at 0.3 µg/ml + Human Amygdala lysates at 35 µg
Developed using the ECL technique.
Predicted band size: 96 kDa
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab10149 は 3 報の論文で使用されています。
- Conte IL et al. Interaction between MyRIP and the actin cytoskeleton regulates Weibel-Palade body trafficking and exocytosis. J Cell Sci 129:592-603 (2016). PubMed: 26675235
- Song J et al. TLR4-mediated expulsion of bacteria from infected bladder epithelial cells. Proc Natl Acad Sci U S A 106:14966-71 (2009). IP ; Human . PubMed: 19706440
- Nightingale TD et al. Rab27a and MyRIP regulate the amount and multimeric state of VWF released from endothelial cells. Blood 113:5010-8 (2009). ICC/IF ; Human . PubMed: 19270261
