Anti-Myosin VIIa/MYO7A 抗体 (ab3481)
Key features and details
- Rabbit polyclonal to Myosin VIIa/MYO7A
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Guinea pig, Cow, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Myosin VIIa/MYO7A antibody
Myosin VIIa/MYO7A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Myosin VIIa/MYO7A -
由来種
Rabbit -
特異性
Detects Myosin VIIa/MYO7A from mouse tissues as well as recombinant. By Western blot, this antibody detects an ~220 kDa protein representing myosin VIIa/MYO7A from mouse testes preparations. This antibody detects recombinant mouse myosin VIIa/MYO7A overexpressed in Sf9 insect cell lysate.
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アプリケーション
適用あり: Flow Cyt, WBmore details -
種交差性
交差種: Mouse, Guinea pig, Cow, Human -
免疫原
Synthetic peptide corresponding to Mouse Myosin VIIa/MYO7A aa 16-31.
Sequence:SGQEFDVPIGAVVKLC
(Peptide available asab4996) -
特記事項
This product was previously labelled as Myosin VIIa
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
一次抗体 備考
Myosin VIIa is a member of the myosin superfamily of actin-based motor proteins. Defects in the myosin VIIa gene are responsible for hearing impairment in shaker-1 (sh1) mice and causes Usher syndrome IB in humans. Usher syndrome associates congenital deafness, vestibular dysfunction, and retinitis pigmentosa and is the most common form of combined deafness and blindness. Structural features of myosin VIIa protein include an ATP binding N-terminal motor domain, a central region which possess five light-chain binding (IQ) motifs, and a C-terminal domain with three myosin tail homology (MyTH4) and talin-like homology regions. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab3481 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt | Use 3-5µg for 106 cells. | |
| WB | Use a concentration of 5 µg/ml. Detects a band of approximately 220 kDa. |
ターゲット情報
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関連性
Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. In retina, myosin VIIa may play a role in trafficking of ribbon-synaptic vesicle complexes and renewal of the outer photoreceptors disks. In inner ear, it may maintain the rigidity of stereocilia during the dynamic movements of the bundle. -
細胞内局在
Cytoplasmic, cytoskeleton -
参照データベース
- Entrez Gene: 4647 Human
- Entrez Gene: 17921 Mouse
- Omim: 276903 Human
- SwissProt: Q13402 Human
- SwissProt: P97479 Mouse
- Unigene: 370421 Human
- Unigene: 1403 Mouse
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別名
- Deafness autosomal dominant 11 antibody
- Deafness autosomal recessive 2 antibody
- DFNA11 antibody
see all
画像
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Western blot - Anti-Myosin VIIa/MYO7A antibody (ab3481)Anti-Myosin VIIa/MYO7A antibody (ab3481) at 5 µg/ml + Membrane enriched extracts of L6 at 30 µg with Skimmed milk at 5 %
Secondary
HRP conjugated goat anti-rabbit IgG (H+L) at 1/4000 dilution -
Western blot - Anti-Myosin VIIa/MYO7A antibody (ab3481)Western blot detection of Myosin VIIa/MYO7A in mouse testes tissue extract using ab3481.
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Flow Cytometry - Anti-Myosin VIIa/MYO7A antibody (ab3481)ab3481 staining Myosin VIIa/MYO7A (red histogram) on L6 cells by Flow Cytometry. Cells were fixed with 70% ethonal, permeabilized with Triton and blocked with 5% BSA for 30 minutes at room temperature. The sample was incubated with the primary antibody (3-5 ug/10^6 in 2.5% BSA) for 2 hours at room temperature. An Alexa fluor® 488-conjugated Goat anti-rabbit IgG (1/400) was used as the secondary antibody. Purple histogram represents unstained control, pink histogram represents rabbit isotype control and green histogram represents no primary antibody control
プロトコール
参考文献 (16)
ab3481 は 16 報の論文で使用されています。
- Mei H et al. Increased mitochondrial DNA copy number protects hair cells and HEI-OC1 cells against drug-induced apoptosis. Mol Med Rep 21:338-346 (2020). PubMed: 31939628
- Xu W et al. Endothelial FAM3A positively regulates post-ischaemic angiogenesis. EBioMedicine 43:32-42 (2019). PubMed: 31000420
- Liu Y et al. Unconventional myosin VIIA promotes melanoma progression. J Cell Sci 131:N/A (2018). PubMed: 29361540
- Fan F et al. Exophilin-8 assembles secretory granules for exocytosis in the actin cortex via interaction with RIM-BP2 and myosin-VIIa. Elife 6:N/A (2017). PubMed: 28673385
- Santos AC et al. Cochlear epithelial of dog fetuses: a new source of multipotent stem cells. Cytotechnology 69:179-189 (2017). PubMed: 28074389
