Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5335] to Myosin, smooth muscle heavy chain 1 and 2 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
製品名Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] - BSA and Azide free
Myosin, smooth muscle heavy chain 1 and 2 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR5335] to Myosin, smooth muscle heavy chain 1 and 2 - BSA and Azide free
アプリケーション適用あり: IHC-P, WBmore details
種交差性交差種: Mouse, Human
Synthetic peptide within Human Myosin, smooth muscle heavy chain 1 and 2. The exact sequence is proprietary.
ab247958 is the carrier-free version of ab124679 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247958 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
解離定数（KD 値）KD = 1.85 x 10 -10 M Learn more about KD
Concentration information loading...
精製度Protein A purified
Our Abpromise guarantee covers the use of ab247958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 250 kDa (predicted molecular weight: 227 kDa).|
組織特異性Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea.
関連疾患Note=A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11.
Defects in MYH11 are the cause of aortic aneurysm familial thoracic type 4 (AAT4) [MIM:132900]; also known as familial thoracic aortic aneurysm and dissection (TAAD). Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Patients with AAT4 show marked aortic stiffness. Pathological aortas show large areas of medial degeneration with very low smooth muscle cells content.
配列類似性Contains 1 IQ domain.
Contains 1 myosin head-like domain.
ドメインThe rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
細胞内局在Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils.
- Information by UniProt
- AAT4 antibody
- DKFZp686D10126 antibody
- DKFZp686D19237 antibody
All lanes : Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679) at 1/10000 dilution (unpurified)
Lane 1 : Human fetal artery lysate
Lane 2 : Human bladder lysate
Lane 3 : Mouse spleen lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 227 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
This data was developed using ab124679, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.