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Signal Transduction Cytoskeleton / ECM Cytoskeleton Motor Proteins Myosin
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Anti-Myosin light chain (phospho S20) 抗体 (ab2480)

  • Datasheet
  • SDS
Reviews (15)Q&A (16)References (100)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-Myosin light chain (phospho S20) antibody (ab2480)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain (phospho S20) antibody (ab2480)
  • Western blot - Anti-Myosin light chain (phospho S20) antibody (ab2480)

Key features and details

  • Rabbit polyclonal to Myosin light chain (phospho S20)
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human, Recombinant fragment
  • Isotype: IgG

こちらの製品もご検討ください

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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
ライゼート
C2C12 whole cell lysate (ab7182)
一次抗体
Product image
Anti-Myosin Light Chain 2 antibody [AT3B2] (ab89594)

関連製品

製品の概要

  • 製品名

    Anti-Myosin light chain (phospho S20) antibody
    Myosin light chain 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Myosin light chain (phospho S20)
  • 由来種

    Rabbit
  • 特異性

    This phospho specific polyclonal antibody is specific for phosphorylated pS20 of human myosin light chain. Reactivity with non-phosphorylated human myosin light chain is less than 1% by ELISA. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.

    Customers have seen variable performance with Xenopus laevis.

  • アプリケーション

    適用あり: WB, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Human, Recombinant fragment
  • 免疫原

    Human Myosin Light Chain phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH) - KKRPQRAT(pS)NVFAMFD (aa 12-27).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    This antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Myosin
    • Stem Cells
    • Mesenchymal Stem Cells
    • Myogenesis
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Myosins

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab2480の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (7)
1/500 - 1/2000. Predicted molecular weight: 20 kDa.

We recommend using BSA for blocking when using phospho-specific antibodies.

IHC-P (2)
Use a concentration of 2.5 µg/ml.
特記事項
WB
1/500 - 1/2000. Predicted molecular weight: 20 kDa.

We recommend using BSA for blocking when using phospho-specific antibodies.

IHC-P
Use a concentration of 2.5 µg/ml.

ターゲット情報

  • 機能

    Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
  • 組織特異性

    Smooth muscle tissues and in some, but not all, nonmuscle cells.
  • 配列類似性

    Contains 3 EF-hand domains.
  • 翻訳後修飾

    Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.
  • Target information above from: UniProt accession P24844 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 10398 Human
    • Entrez Gene: 98932 Mouse
    • Omim: 609905 Human
    • SwissProt: P24844 Human
    • SwissProt: Q9CQ19 Mouse
    • Unigene: 504687 Human
    • Unigene: 271770 Mouse
    • 別名

      • 20 kDa myosin light chain antibody
      • KMLC antibody
      • LC20 antibody
      • MLC-2C antibody
      • MLCK2 antibody
      • MRLC1 antibody
      • MYL9 antibody
      • MYL9_HUMAN antibody
      • Myosin regulatory light chain 2 antibody
      • Myosin regulatory light chain 2, smooth muscle isoform antibody
      • Myosin regulatory light chain 9 antibody
      • Myosin regulatory light chain MRLC1 antibody
      • Myosin regulatory light polypeptide 9 antibody
      • Myosin RLC antibody
      • Myosin, light chain 9, regulatory antibody
      • MYRL2 antibody
      • smooth muscle isoform antibody
      see all

    画像

    • Western blot - Anti-Myosin light chain (phospho S20) antibody (ab2480)
      Western blot - Anti-Myosin light chain (phospho S20) antibody (ab2480)
      All lanes : Anti-Myosin light chain (phospho S20) antibody (ab2480) at 1/1000 dilution

      Lane 1 : Regulatory Light Chain Non-Phospho recombinant protein, 50 ng
      Lane 2 : Regulatory Light Chain Phospho recombinant protein, 50 ng
      Lane 3 : Smooth Muscle Non-Phospho recombinant protein, 50 ng
      Lane 4 : Smooth Muscle Phospho recombinant protein, 50 ng

      Secondary
      All lanes : Peroxidase rabbit secondary antibody at 1/40000 dilution

      Predicted band size: 20 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain (phospho S20) antibody (ab2480)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain (phospho S20) antibody (ab2480)

      IHC-P of ab2480 at 2.5 µg/ml staining both vascular and myometrial smooth muscle cells of the human uterus. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

    • Western blot - Anti-Myosin light chain (phospho S20) antibody (ab2480)
      Western blot - Anti-Myosin light chain (phospho S20) antibody (ab2480)

      Rabbit polyclonal to phospho Myosin Light Chain phospho Myosin Light Chain (Ser 20) (ab2480) used at a 1/5000 dilution to detect myosin light chain by Western blot. Either 13 ug (lanes 1-3) or 20 ug (lanes 4-7) of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE.  Samples were either mock-treated or CLA treated:
      Lane 1 : untreated 45 min
      Lane 2 : CLA 50 nm 45 min
      Lane 3 : CLA 100 nm 45 min
      Lane 4 : A21 untreated 45 min
      Lane 5 : A22 A23187 5 min
      Lane 6 : A23 A23187 15 min
      Lane 7 : A24 A23187 60 min
      After washing, a 1/5,000 dilution of anti-rabbit HRP (ab7090) was used as secondary.

      Rabbit polyclonal to phospho Myosin Light Chain phospho Myosin Light Chain (Ser 20) (ab2480) used at a 1/5000 dilution to detect myosin light chain by Western blot. Either 13 ug (lanes 1-3) or 20 ug (lanes 4-7) of a mouse cardiac myocyte lysate was loaded on a 4-20

    プロトコール

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (100)

    ab2480 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2480 は 100 報の論文で使用されています。

    • Ding J  et al. Myosin light chain kinase inhibitor ML7 improves vascular endothelial dysfunction and permeability via the mitogen-activated protein kinase pathway in a rabbit model of atherosclerosis. Biomed Pharmacother 128:110258 (2020). PubMed: 32516749
    • Guo C  et al. Bilobalide reversibly modulates blood-brain barrier permeability through promoting adenosine A1 receptor-mediated phosphorylation of actin-binding proteins. Biochem Biophys Res Commun 526:1077-1084 (2020). PubMed: 32312522
    • Meyer K  et al. Bile canaliculi remodeling activates YAP via the actin cytoskeleton during liver regeneration. Mol Syst Biol 16:e8985 (2020). PubMed: 32090478
    • Yang H  et al. Effects of dexmedetomidine on glioma cells in the presence or absence of cisplatin. J Cell Biochem 121:723-734 (2020). PubMed: 31452248
    • Evans F  et al. Signaling pathways in cytoskeletal responses to plasma membrane depolarization in corneal endothelial cells. J Cell Physiol 235:2947-2962 (2020). PubMed: 31535377
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 31 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Myosin light chain (phospho S20) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U87-MG)
    Permeabilization
    Yes - 0.5% triton
    Specification
    U87-MG
    Blocking step
    Goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 02 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Myosin light chain (phospho S20) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Dog Cell (MDCK)
    Permeabilization
    Yes - 0.2% Triton X100
    Specification
    MDCK
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 11 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myosin light chain (phospho S20) antibody

    Good
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (head)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA based buffer pH 7.8
    Permeabilization
    No
    Specification
    head
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 13 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myosin light chain (phospho S20) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Prostate)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate, pH 6
    Permeabilization
    No
    Specification
    Prostate
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 28 2017

    IHC - Wholemount abreview for Anti-Myosin light chain (phospho S20) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Hydra vulgaris Tissue (morphogenesis)
    Specification
    morphogenesis
    Read More

    Mr. Oliver Holz

    Verified customer

    投稿 Jul 21 2017

    Western blot abreview for Anti-Myosin light chain (phospho S20) antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Intestinal tissue)
    Gel Running Conditions
    Reduced Denaturing (4-12% BIS TRIS)
    Loading amount
    15 µg
    Specification
    Intestinal tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 08 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Myosin light chain (phospho S20) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human arterial myocytes)
    Specification
    Human arterial myocytes
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 08 2015

    Western blot

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (15)
    Sample
    Human Tissue lysate - whole (Heart)
    Specification
    Heart
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 May 22 2013

    Question

    Do you have any myosin light chain antibodies that detect both the phosphorylated and non-phosphorylated bands on a western blot? I have ab11082 which someone told me is able to detect both bands, but I have had no success so far. The best I can do is blot with ab11082 and then with ab2480 to get the phosphorylated band, but I am wanting to compare the bands and would prefer just one antibody for accuracy.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 20 2012

    Answer

    Thank you for contacting us.

    Are you looking for a specific light chain (i.e. 2 or 3)? We only have 2 other anti-myosin light chain antibodies as ab97891 and ab88726 but we don't have any WB images showing 2 bands for phopsho and unphosphorylated protein. You may have to do a WB with a phospho-specific antibody if you want to see clear distinctions between phosphorylated and total proteins.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Dec 20 2012

    Question

    Does that mean that Abcam didn't test the
    cross reactivity of this antibody to skeletal muscle isoform of
    phosphorylated myosin regulatory light chain? Could you also let me know by
    what means you found that this antibody doesn't cross react with cardiac isoform?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 06 2012

    Answer



    You are correct that Abcam has not specifically tested cross reactivity of ab2480 with skeletal muscle isoform MLC. This cross reactivity is predicted based on similarity of the immunogen sequence to skeletal muscle MLC. Likewise, ab2480 has not been tested for reactivity to cardiac MLC but the immunogen sequence does not exist in the cardiac MLC.

    Read More

    Abcam Scientific Support

    Answered on Dec 06 2012

    1-10 of 31 Abreviews or Q&A

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