Anti-Myoglobin 抗体 [EP3081Y] (ab77232)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP3081Y] to Myoglobin
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Myoglobin antibody [EP3081Y]
Myoglobin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP3081Y] to Myoglobin -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Myoglobin aa 100-200 (C terminal). The exact sequence is proprietary.
Database link: P02144 -
ポジティブ・コントロール
- Human, rat and mouse muscle tissue lysates; human heart muscle tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
解離定数(KD 値)
KD = 1.78 x 10 -10 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP3081Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77232の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 17 kDa.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Use of an HRP/AP polymerized secondary antibody is recommended. See IHC antigen retrieval protocols. |
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ICC/IF |
Use at an assay dependent concentration.
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特記事項 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 17 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Use of an HRP/AP polymerized secondary antibody is recommended. See IHC antigen retrieval protocols. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. -
配列類似性
Belongs to the globin family. - Information by UniProt
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参照データベース
- Entrez Gene: 4151 Human
- Entrez Gene: 17189 Mouse
- Entrez Gene: 59108 Rat
- Omim: 160000 Human
- SwissProt: P02144 Human
- SwissProt: P04247 Mouse
- SwissProt: Q9QZ76 Rat
- Unigene: 517586 Human
see all -
別名
- MB antibody
- MGC13548 antibody
- MYG_HUMAN antibody
- Myoglobin antibody
画像
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All lanes : Anti-Myoglobin antibody [EP3081Y] (ab77232) at 1/5000 dilution (Purified)
Lane 1 : Human muscle lysate with 5% NFDM/TBST
Lane 2 : Mouse muscle lysate with 5% NFDM/TBST
Lane 3 : Rat muscle lysate with 5% NFDM/TBST
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 17 kDa
Observed band size: 17 kDa
Exposure time: 1 second -
Purified ab77232 staining Myoglobin in Human cardiac muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a Tris/EDTA buffer (ab93684 Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/1000). A HRP-conjugated Goat Anti-Rabbit IgG H&L (ready to use) was used as the secondary antibody. Counterstained with Hematoxylin.
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Purified ab77232 staining Myoglobin in the C2C12 (mouse myoblasts myoblast) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol. Samples were incubated with primary antibody and an Alexa Fluor® 488-conjugated Goat anti-rabbit IgG (ab150077) was used as the secondary antibody. Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) and DAPI.
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Purified ab77232 staining Myoglobin in Mouse cardiac muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a Tris/EDTA buffer (ab93684 Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/1000). A HRP-conjugated Goat Anti-Rabbit IgG H&L (ready to use) was used as the secondary antibody. Counterstained with Hematoxylin.
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Purified ab77232 staining Myoglobin in Rat cardiac muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a Tris/EDTA buffer (ab93684 Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/1000). A HRP-conjugated Goat Anti-Rabbit IgG H&L (ready to use) was used as the secondary antibody. Counterstained with Hematoxylin.
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Unpurified ab77232 showing positive staining in human Skeletal muscle tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Western blot analysis of Pig skeletal muscle whole tissue lysate (10μg/lane) labelling Myoglobin with unpurified ab77232 at 1/5000 in 1x PBS + 0.1% Tween-20 for 17 hours at 4ºC. A IRDye® 680-conjugated Donkey anti-rabbit polyclonal (1/40000) was used as the secondary antibody.
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All lanes : Anti-Myoglobin antibody [EP3081Y] (ab77232) at 1/5000 dilution (Unpurified)
Lane 1 : Human heart muscle lysate
Lane 2 : Mouse heart muscle lysate
Lane 3 : Rat heart muscle lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa -
Unpurified ab77232, at a 1/250 dilution, staining Myoglobin in paraffin embedded human heart muscle by Immunohistochemistry.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Unpurified ab77232 showing negative staining in Normal human brain tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Unpurified ab77232 showing negative staining in Normal human kidney tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Unpurified ab77232 showing negative staining in Normal human liver tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Produced using unpurified ab77232.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (29)
ab77232 は 29 報の論文で使用されています。
- Majerczak J et al. Nitrite Concentration in the Striated Muscles Is Reversely Related to Myoglobin and Mitochondrial Proteins Content in Rats. Int J Mol Sci 23:N/A (2022). PubMed: 35269826
- Liu F et al. HDAC9 exacerbates myocardial infarction via inactivating Nrf2 pathways. J Pharm Pharmacol N/A:N/A (2021). PubMed: 33963859
- Blackburn ML et al. On the potential role of globins in brown adipose tissue: a novel conceptual model and studies in myoglobin knockout mice. Am J Physiol Endocrinol Metab 321:E47-E62 (2021). PubMed: 33969705
- Elsherbiny ME et al. Expression of Myoglobin in Normal and Cancer Brain Tissues: Correlation With Hypoxia Markers. Front Oncol 11:590771 (2021). PubMed: 33996536
- Ono-Moore KD et al. Metabolic physiology and skeletal muscle phenotypes in male and female myoglobin knockout mice. Am J Physiol Endocrinol Metab 321:E63-E79 (2021). PubMed: 33969704