Application
Western blot
Sample
Human Cell lysate - whole cell (skeletal myotubes)
Loading amount
5 µg
Specification
skeletal myotubes
Gel Running Conditions
Reduced Denaturing (10 % gel, semi-dry)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Dilution
1/1000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS with 0.1% Tween
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2500
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 34 kDa Non-specific: several from 25 to 8 kDa
Positive control
skeletal myotubes
Additional data
Additional Notes
marker on image from top to bottom: 83, 62, 47, 32, 25 and 16 kD
Abcam response
Thank you for your honest feedback. This western blot image looks similar to that seen in an abreview for another anti-Myogenin antibody, ab11986.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Henrike Sell
Verified customer
投稿 Feb 02 2010