Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1457] to MYL12A (phospho T17 + S18) - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
製品名Anti-MYL12A (phospho T17 + S18) antibody [EPR1457] - BSA and Azide free
MYL12A 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR1457] to MYL12A (phospho T17 + S18) - BSA and Azide free
This antibody only detects MYL12A phosphorylated at Threonine 17 and Serine 18.
アプリケーション適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human MYL12A (phospho S17 + S18). The exact sequence is proprietary.
Database link: P19105
Ab248158 is the carrier-free version of ab126739. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248158 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
Concentration information loading...
Our Abpromise guarantee covers the use of ab248158 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa).|
機能Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
配列類似性Contains 3 EF-hand domains.
翻訳後修飾Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.
- Information by UniProt
- ML12A_HUMAN antibody
- MLC 2B antibody
- MLC-2B antibody
All lanes : Anti-MYL12A (phospho T17 + S18) antibody [EPR1457] (ab126739) at 1/1000 dilution
Lane 1 : NIH/3T3 cell lysate
Lane 2 : NIH/3T3 cell lysate + Calyculin A
Lysates/proteins at 10 µg per lane.
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 20 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
This data was developed using ab126739, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.