Anti-MYH2 抗体 [EPR5280] - BSA and Azide free (ab248064)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5280] to MYH2 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-MYH2 antibody [EPR5280] - BSA and Azide free
MYH2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5280] to MYH2 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
ab248064 is the carrier-free version of ab124937.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
解離定数(KD 値)
KD = 6.90 x 10 -11 M Learn more about KD -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5280 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248064の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 220 kDa (predicted molecular weight: 223 kDa).
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特記事項 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 220 kDa (predicted molecular weight: 223 kDa). |
ターゲット情報
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機能
Muscle contraction. Required for cytoskeleton organization. -
関連疾患
Defects in MYH2 are the cause of inclusion body myopathy type 3 (IBM3) [MIM:605637]. Hereditary inclusion body myopathies constitute a group of neuromuscular disorders characterized by slowly progressive distal and proximal weakness and a typical muscle pathology including rimmed vacuoles and filamentous inclusions. IBM3 is a variant of hereditary inclusion body myopathies and is characterized by autosomal dominant myopathy with joint contracture, ophthalmoplegia and rimmed vacuoles. Morphological analysis of muscle biopsies from patients indicate that the type 2A fibers frequently were abnormal, whereas other fiber types appeared normal. -
配列類似性
Contains 1 IQ domain.
Contains 1 myosin head-like domain. -
ドメイン
The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2). -
細胞内局在
Cytoplasm > myofibril. Thick filaments of the myofibrils. - Information by UniProt
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参照データベース
- Entrez Gene: 4620 Human
- Entrez Gene: 17882 Mouse
- Entrez Gene: 691644 Rat
- Omim: 160740 Human
- SwissProt: Q9UKX2 Human
- SwissProt: Q5SX41 Mouse
- SwissProt: Q07443 Rat
- Unigene: 667534 Human
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別名
- adult 2 antibody
- Fast 2a myosin heavy chain antibody
- IBM3 antibody
see all
画像
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All lanes : Anti-MYH2 antibody [EPR5280] (ab124937) at 1/10000 dilution
Lane 1 : Mouse muscle tissue lysate
Lane 2 : Rat muscle tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 223 kDa
Observed band size: 220 kDa why is the actual band size different from the predicted?This data was developed using ab124937, the same antibody clone in a different buffer formulation.
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Anti-MYH2 antibody [EPR5280] (ab124937) at 1/10000 dilution + Human trachea lysate at 10 µg
Secondary
HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 223 kDa
Observed band size: 220 kDa why is the actual band size different from the predicted?This data was developed using ab124937, the same antibody clone in a different buffer formulation.
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This data was developed using ab124937, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab248064 は論文での使用が確認できていません。