Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Intestine, Spleen)
Permeabilization
No
Specification
Intestine, Spleen
Blocking step
10% Normal Serum, 1% BSA, 0.3M Glycine in TBS as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in TBS
Dilution
1/100
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Invitrogen A21206 Donkey Anti Rabbit IgG AF488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
PubMed ID
30336264: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Brett Bell
Verified customer
投稿 Oct 21 2020