Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Oligodendroglia)
Specification
Oligodendroglia
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.01% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Other product details
Dilution
1/100
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% horse serum in PBS + 0.01% TX100
Secondary antibody
Name
Non-Abcam antibody was used: Rabbit-anti-mouse (IgG2a)-TexasRed
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Texas Red ®
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Texas Red ®
Dilution
1/100
Additional data
Additional Notes
The use of this antibody for ICC/IF for rat primary oligodendroglia staining is not bad, although I believe the are antibodies which provide better staining of the oligodendroglial membrane sheets. I've included co-staining with an anti-GalC (galactosylcerebroside) antibody as a positive control that the antibody is staining oligodendroglia.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Sep 07 2007