Application
Western blot
Sample
Mouse Tissue lysate - whole (liver and brain)
Gel Running Conditions
Reduced Denaturing (4-20)
Loading amount
10 µg
Specification
liver and brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: anti-Rabbit IgG HRP linked (cell signaling)
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL prime
Negative control
liver
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 28 kDa
Positive control
brain
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Isabelle Cote
Verified customer
投稿 Sep 28 2017