Name: Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281]
Brand: RabMAb
SKU: ab109746
Rating: 4 (6 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP4281] to Myelin oligodendrocyte glycoprotein
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Alexa Fluor® 647 Carrier Free HRP

製品名

Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281]
Myelin oligodendrocyte glycoprotein 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EP4281] to Myelin oligodendrocyte glycoprotein
由来種

Rabbit
アプリケーション

適用あり: WB, IHC-Pmore details
種交差性

交差種: Mouse, Rat, Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- Mouse brain, Rat brain and Human brain lysates; Human astrocytoma tissue and Human brain tissue
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EP4281
アイソタイプ

IgG
研究分野
- Neuroscience
- Development

Alternative Versions
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Rat brain normal tissue lysate - membrane extract (ab29473)
Recombinant Protein
- Recombinant Human Myelin oligodendrocyte glycoprotein (ab152080)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab109746の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB	(3)	1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
IHC-P		1/1000 - 1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols.

特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols.

機能

Mediates homophilic cell-cell adhesion (By similarity). Minor component of the myelin sheath. May be involved in completion and/or maintenance of the myelin sheath and in cell-cell communication.
組織特異性

Found exclusively in the CNS, where it is localized on the surface of myelin and oligodendrocyte cytoplasmic membranes.
配列類似性

Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
細胞内局在

Cell membrane.
Target information above from: UniProt accession Q16653 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 4340 Human
- Entrez Gene: 17441 Mouse
- Entrez Gene: 24558 Rat
- Omim: 159465 Human
- SwissProt: Q16653 Human
- SwissProt: Q61885 Mouse
- SwissProt: Q63345 Rat
- Unigene: 141308 Human
- Unigene: 210857 Mouse
- Unigene: 9687 Rat
see all
別名
- BTN6 antibody
- BTNL11 antibody
- MGC26137 antibody
- MOG alpha 5 antibody
- MOG alpha 6 antibody
- MOG AluA antibody
- MOG AluB antibody
- MOG antibody
- MOG Ig AluB antibody
- MOG_HUMAN antibody
- MOGIG2 antibody
- Myelin oligodendrocyte glycoprotein antibody
- Myelin-oligodendrocyte glycoprotein antibody
- NRCLP7 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling Myelin oligodendrocyte glycoprotein with purified ab109746 at 1:1000 dilution (1.12 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.
Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

All lanes : Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : Human brain lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 28 kDa

This image was produced using unpurified antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling Myelin oligodendrocyte glycoprotein with Purified ab109746 at 1:1000 dilution (1.12 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Myelin oligodendrocyte glycoprotein with purified ab109746 at 1:1000 dilution (1.12 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human astrocytoma tissue sections labeling Myelin oligodendrocyte glycoprotein with purified ab109746 at 1:1000 dilution (1.12 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.
Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746) at 1/2000 dilution (Purified) + Mouse brain lysates at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 28 kDa
Observed band size: 28 kDa
Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

All lanes : Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746) at 1/2000 dilution

Lane 1 : Human brain lysates
Lane 2 : Rat brain lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 28 kDa
Observed band size: 28 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

ab109746, at 1/1000 dilution, staining Myelin oligodendrocyte glycoprotein in paraffin-embedded Human astrocytoma tissue by Immunohistochemistry. This image was produced using unpurified antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

ab109746, at 1/1000 dilution, staining Myelin oligodendrocyte glycoprotein in paraffin-embedded Human brain tissue by Immunohistochemistry. This image was produced using unpurified antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

ab109746 showing negative staining in Breast carcinoma tissue. This image was produced using unpurified antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

ab109746 showing negative staining in Normal breast tissue. This image was produced using unpurified antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

ab109746 showing positive staining in Glioblastoma tissue. This image was produced using unpurified antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

ab109746 showing negative staining in Colonic adenocarcinoma tissue. This image was produced using unpurified antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

ab109746 showing negative staining in Normal tonsil tissue. This image was produced using unpurified antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

ab109746 showing negative staining in Normal colon tissue. This image was produced using unpurified antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

ab109746 showing negative staining in Benign prostatic hyperplasia tissue. This image was produced using unpurified antibody.
Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

Western blot protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab109746 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab109746 は 14 報の論文で使用されています。

Yoo IH et al. The Anti-Inflammatory Effect of Sulforaphane in Mice with Experimental Autoimmune Encephalomyelitis. J Korean Med Sci 34:e197 (2019). PubMed: 31327180
Wang J et al. Glioblastoma extracellular vesicles induce the tumour-promoting transformation of neural stem cells. Cancer Lett N/A:N/A (2019). PubMed: 31521694
Trinh QD et al. Myelin Oligodendrocyte Glycoprotein-Independent Rubella Infection of Keratinocytes and Resistance of First-Trimester Trophoblast Cells to Rubella Virus In Vitro. Viruses 10:N/A (2018). PubMed: 29300335
Jana M et al. Upregulation of Myelin Gene Expression by a Physically-Modified Saline via Phosphatidylinositol 3-Kinase-Mediated Activation of CREB: Implications for Multiple Sclerosis. Neurochem Res 43:407-419 (2018). PubMed: 29143164
Duan C et al. Sulfasalazine alters microglia phenotype by competing endogenous RNA effect of miR-136-5p and long non-coding RNA HOTAIR in cuprizone-induced demyelination. Biochem Pharmacol 155:110-123 (2018). PubMed: 29944870

View all Publications for this product

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-6 of 6 Abreviews or Q&A

Application

Western blot

Sample

Mouse Tissue lysate - whole (Brain)

Gel Running Conditions

Non-reduced Denaturing (4-20%)

Loading amount

15 µg

Specification

Brain

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

投稿 Jan 11 2022

Application

Immunohistochemistry free floating

Sample

Mouse Tissue sections (Brain cortex)

Specification

Brain cortex

Abcam user community

Verified customer

投稿 Sep 10 2020

Application

Immunocytochemistry/ Immunofluorescence

Sample

Rat Cell (oligodendrocyte cell line OLN93)

Permeabilization

Specification

oligodendrocyte cell line OLN93

Blocking step

BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 4% · Temperature: 25°C

Fixative

Formaldehyde

Abcam user community

Verified customer

投稿 Aug 06 2018

Application

Western blot

Sample

Rat Tissue lysate - whole (liver and brain)

Gel Running Conditions

Reduced Denaturing (12.5%)

Loading amount

50 µg

Specification

liver and brain

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

投稿 Nov 13 2017

Application

Western blot

Sample

Mouse Tissue lysate - whole (liver and brain)

Gel Running Conditions

Reduced Denaturing (4-20)

Loading amount

10 µg

Specification

liver and brain

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Isabelle Cote

Verified customer

投稿 Sep 28 2017

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (Primary oligodendrocytes differentiated for 5 days)

Permeabilization

Specification

Primary oligodendrocytes differentiated for 5 days

Blocking step

Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

投稿 May 05 2017

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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (14)

レビューと Q&A

Western blot abreview for Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281]

Immunohistochemistry free floating abreview for Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281]

Western blot abreview for Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281]

Western blot abreview for Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281]