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Anti-Myelin Basic Protein 抗体 [12] (ab7349)

  • Datasheet
  • SDS
Reviews (20)Q&A (20)References (204)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-Myelin Basic Protein antibody [12] (ab7349)
  • Western blot - Anti-Myelin Basic Protein antibody [12] (ab7349)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] (ab7349)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] (ab7349)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [12] to Myelin Basic Protein
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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HRP Anti-Myelin Basic Protein antibody (ab77895)

関連製品

製品の概要

  • 製品名

    Anti-Myelin Basic Protein antibody [12]
    Myelin Basic Protein 一次抗体 製品一覧
  • 製品の詳細

    Rat monoclonal [12] to Myelin Basic Protein
  • 由来種

    Rat
  • アプリケーション

    適用あり: WB, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Rat, Human
  • 免疫原

    Full length protein corresponding to Cow Myelin Basic Protein.

  • エピトープ

    Amino acids 82-87 (DENPVV).
  • ポジティブ・コントロール

    • WB: Human cerebellum, Mouse brain tissue and Rat brain tissue. IHC-P: Human cerebellum tissue.
  • 特記事項

    This product has switched from a hybridoma to recombinant production method on 08th November 2021.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • 精製度

    Ion Exchange Chromatography
  • ポリ/モノ

    モノクローナル
  • クローン名

    12
  • ミエローマ

    NS0
  • アイソタイプ

    IgG2a
  • 研究分野

    • Neuroscience
    • Cell Adhesion Proteins
    • Membrane Proteins
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Oligodendrocyte marker
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Glial
    • Neuroscience
    • Development

関連製品

  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    • Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Isotype control

    • Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)
  • Recombinant Protein

    • Recombinant Human Myelin Basic Protein (Tagged) (ab43614)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab7349の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (2)
1/1000.
IHC-P (5)
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
WB
1/1000.
IHC-P
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
  • 組織特異性

    MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
  • 関連疾患

    Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
  • 配列類似性

    Belongs to the myelin basic protein family.
  • 発生段階

    Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
  • 翻訳後修飾

    Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
    The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
    Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
  • 細胞内局在

    Myelin membrane. Cytoplasmic side of myelin.
  • Target information above from: UniProt accession P02686 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 4155 Human
    • Entrez Gene: 17196 Mouse
    • Entrez Gene: 24547 Rat
    • Omim: 159430 Human
    • SwissProt: P02686 Human
    • SwissProt: P04370 Mouse
    • SwissProt: P02688 Rat
    • Unigene: 551713 Human
    • Unigene: 252063 Mouse
    • Unigene: 392350 Mouse
    • Unigene: 454459 Mouse
    see all
  • 別名

    • GDB antibody
    • Golli MBP antibody
    • Golli MBP; myelin basic protein antibody
    • Hemopoietic MBP antibody
    • HMBPR antibody
    • HUGO antibody
    • MBP antibody
    • MBP_CAVPO antibody
    • MBP_HUMAN antibody
    • MGC99675 antibody
    • MLD antibody
    • Myelin A1 protein antibody
    • Myelin A1 Protein, basic antibody
    • Myelin basic protein antibody
    • Myelin Deficient antibody
    • Myelin membrane encephalitogenic protein antibody
    • OTTHUMP00000163776 antibody
    • OTTHUMP00000174387 antibody
    • OTTHUMP00000174388 antibody
    • SHI antibody
    • Shiverer antibody
    • SP antibody
    see all

画像

  • Western blot - Anti-Myelin Basic Protein antibody [12] (ab7349)
    Western blot - Anti-Myelin Basic Protein antibody [12] (ab7349)
    Anti-Myelin Basic Protein antibody [12] (ab7349) at 1/5000 dilution + Human cerebellum tissue lysate at 10 µg

    Secondary
    Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

    Developed using the ECL technique.

    Observed band size: 18.5 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    The molecular weight observed was in consistency with the literature (PMID: 9299539).

    Blocking buffer: 5% NFDM/TBST.

  • Western blot - Anti-Myelin Basic Protein antibody [12] (ab7349)
    Western blot - Anti-Myelin Basic Protein antibody [12] (ab7349)
    All lanes : Anti-Myelin Basic Protein antibody [12] (ab7349) at 1/5000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

    Observed band size: 14-21.5 kDa why is the actual band size different from the predicted?



    The molecular weight observed was in consistency with the literature (PMID: 9299539).

    Blocking buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 3 seconds; Lane 2: 1 second.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] (ab7349)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] (ab7349)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum labeling Myelin Basic Protein with ab7349 at 1/4000 dilution (0.259 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer; ab214882). The section was incubated with ab7349 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] (ab7349)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] (ab7349)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum labeling Myelin Basic Protein with ab7349 at 1/4000 dilution (0.259 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer; ab214882). The section was incubated with ab7349 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with DAPI.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

プロトコール

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (204)

ab7349 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7349 は 204 報の論文で使用されています。

  • Wu S  et al. Retinoic acid increases phagocytosis of myelin by macrophages. J Cell Physiol 236:3929-3945 (2021). PubMed: 33165955
  • Melero-Jerez C  et al. Myeloid-derived suppressor cells support remyelination in a murine model of multiple sclerosis by promoting oligodendrocyte precursor cell survival, proliferation, and differentiation. Glia 69:905-924 (2021). PubMed: 33217041
  • Iacono D  et al. Reduction of pTau and APP levels in mammalian brain after low-dose radiation. Sci Rep 11:2215 (2021). PubMed: 33500491
  • Maas DA  et al. Antioxidant treatment ameliorates prefrontal hypomyelination and cognitive deficits in a rat model of schizophrenia. Neuropsychopharmacology 46:1161-1171 (2021). PubMed: 33564104
  • Boutary S  et al. Squalenoyl siRNA PMP22 nanoparticles are effective in treating mouse models of Charcot-Marie-Tooth disease type 1 A. Commun Biol 4:317 (2021). PubMed: 33750896
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 40 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-Myelin Basic Protein antibody [12]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain (cortex))
Permeabilization
No
Specification
Brain (cortex)
Blocking step
(agent) for 15 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Acetone
Read More

Abcam user community

Verified customer

投稿 Oct 11 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myelin Basic Protein antibody [12]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More

Mr. Hannes Kaddatz

Verified customer

投稿 Nov 13 2020

Immunohistochemistry (Frozen sections) abreview for Anti-Myelin Basic Protein antibody [12]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (brain)
Permeabilization
No
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Acetone
Read More

Abcam user community

Verified customer

投稿 Nov 12 2020

Western blot abreview for Anti-Myelin Basic Protein antibody [12]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
30 µg
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Aug 10 2020

Immunohistochemistry (Frozen sections) abreview for Anti-Myelin Basic Protein antibody [12]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Permeabilization
Yes - Tween-20
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Aug 06 2020

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Myelin Basic Protein antibody [12]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Monkey Tissue sections (brain)
Antigen retrieval step
None
Permeabilization
Yes - triton 1%
Specification
brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 20°C
Fixative
Paraformaldehyde
Read More

MRS. Francoise Geffroy

Verified customer

投稿 Apr 30 2020

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Myelin Basic Protein antibody [12]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (BRAIN)
Antigen retrieval step
None
Permeabilization
Yes - TRITON 075%
Specification
BRAIN
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Temperature: 20°C
Fixative
Paraformaldehyde
Read More

MRS. Francoise Geffroy

Verified customer

投稿 Feb 17 2020

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Myelin Basic Protein antibody [12]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (brain)
Antigen retrieval step
None
Permeabilization
Yes - triton 0.75%
Specification
brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 20°C
Fixative
Paraformaldehyde
Read More

MRS. Francoise Geffroy

Verified customer

投稿 Feb 17 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myelin Basic Protein antibody [12]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA ph 9
Permeabilization
No
Specification
Brain tissue
Blocking step
Normal Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Fixative
Formaldehyde
Read More

Herr Dr. Markus Kipp

Verified customer

投稿 Sep 07 2018

Question

I would appreciate it if you could let me know the accession # of the specific bovine immunogen that has been used to raise the antibody ab7349. Also if you could tell me what the amino acid sequence 82-87 is to which the ab binds, that would be great.

Read More

Abcam community

Verified customer

Asked on Nov 06 2006

Answer

Thank you for your enquiry. I do not have the accession number for the immunogen; however, the amino acid sequence to which the antibody binds is DENPVV. I have updated the datasheet for future reference. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Nov 06 2006

1-10 of 40 Abreviews or Q&A

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