Anti-Myelin Basic Protein 抗体 [12] (ab7349)
Key features and details
- Rat monoclonal [12] to Myelin Basic Protein
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG2a
製品の概要
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製品名
Anti-Myelin Basic Protein antibody [12]
Myelin Basic Protein 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [12] to Myelin Basic Protein -
由来種
Rat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Cow
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免疫原
Full length protein corresponding to Cow Myelin Basic Protein.
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エピトープ
Amino acids 82-87 (DENPVV). -
ポジティブ･コントロール
- WB: Mouse brain tissue lysate. IHC-P: Mouse cerebral cortex tissue.
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特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.1% Sodium azide
Constituent: Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
12 -
ミエローマ
NS0 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab7349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use at an assay dependent concentration. |
ターゲット情報
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機能
The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. -
組織特異性
MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. -
関連疾患
Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease). -
配列類似性
Belongs to the myelin basic protein family. -
発生段階
Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter. -
翻訳後修飾
Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated. -
細胞内局在
Myelin membrane. Cytoplasmic side of myelin. - Information by UniProt
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参照データベース
- Entrez Gene: 618684 Cow
- Entrez Gene: 17196 Mouse
- SwissProt: P02687 Cow
- SwissProt: P04370 Mouse
- Unigene: 252063 Mouse
- Unigene: 392350 Mouse
- Unigene: 454459 Mouse
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別名
- GDB antibody
- Golli MBP antibody
- Golli MBP; myelin basic protein antibody
see all
画像
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![Western blot - Anti-Myelin Basic Protein antibody [12] (ab7349)](https://www.abcam.co.jp/ps/products/7/ab7349/Images/ab7349-12904-anti-myelin-basic-protein-antibody-12-western-blot.gif)
Western blot - Anti-Myelin Basic Protein antibody [12] (ab7349)Anti-Myelin Basic Protein antibody [12] (ab7349) + Mouse brain tissue lysate
Observed band size: 19,26 kDa why is the actual band size different from the predicted?
プロトコール
参考文献 (157)
ab7349 は 157 報の論文で使用されています。
- Tognatta R et al. Astrocytes Are Required for Oligodendrocyte Survival and Maintenance of Myelin Compaction and Integrity. Front Cell Neurosci 14:74 (2020). PubMed: 32300294
- Wedel M et al. Transcription factor Tcf4 is the preferred heterodimerization partner for Olig2 in oligodendrocytes and required for differentiation. Nucleic Acids Res 48:4839-4857 (2020). PubMed: 32266943
- Romero-Ramírez L et al. Treatment of rats with spinal cord injury using human bone marrow-derived stromal cells prepared by negative selection. J Biomed Sci 27:35 (2020). PubMed: 32066435
- Rawji KS et al. Niacin-mediated rejuvenation of macrophage/microglia enhances remyelination of the aging central nervous system. Acta Neuropathol 139:893-909 (2020). PubMed: 32030468
- Maas DA et al. Interneuron hypomyelination is associated with cognitive inflexibility in a rat model of schizophrenia. Nat Commun 11:2329 (2020). PubMed: 32393757
