Key features and details
- Rat monoclonal  to Myelin Basic Protein
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG2a
製品名Anti-Myelin Basic Protein antibody 
Myelin Basic Protein 一次抗体 製品一覧
製品の詳細Rat monoclonal  to Myelin Basic Protein
アプリケーション適用あり: WBmore details
Full length protein corresponding to Cow Myelin Basic Protein.
エピトープAmino acids 82-87 (DENPVV).
- WB: Mouse brain tissue lysate. IHC-P: Mouse cerebral cortex tissue.
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.1% Sodium azide
Constituent: Tissue culture supernatant
Concentration information loading...
精製度Tissue culture supernatant
Our Abpromise guarantee covers the use of ab7349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
機能The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
組織特異性MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
関連疾患Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
配列類似性Belongs to the myelin basic protein family.
発生段階Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
翻訳後修飾Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
細胞内局在Myelin membrane. Cytoplasmic side of myelin.
- Information by UniProt
- GDB antibody
- Golli MBP antibody
- Golli MBP; myelin basic protein antibody
Anti-Myelin Basic Protein antibody  (ab7349) + Mouse brain tissue lysate
Observed band size: 19,26 kDa why is the actual band size different from the predicted?
ab7349 は 157 報の論文で使用されています。
- Tognatta R et al. Astrocytes Are Required for Oligodendrocyte Survival and Maintenance of Myelin Compaction and Integrity. Front Cell Neurosci 14:74 (2020). PubMed: 32300294
- Wedel M et al. Transcription factor Tcf4 is the preferred heterodimerization partner for Olig2 in oligodendrocytes and required for differentiation. Nucleic Acids Res 48:4839-4857 (2020). PubMed: 32266943
- Romero-Ramírez L et al. Treatment of rats with spinal cord injury using human bone marrow-derived stromal cells prepared by negative selection. J Biomed Sci 27:35 (2020). PubMed: 32066435
- Rawji KS et al. Niacin-mediated rejuvenation of macrophage/microglia enhances remyelination of the aging central nervous system. Acta Neuropathol 139:893-909 (2020). PubMed: 32030468
- Maas DA et al. Interneuron hypomyelination is associated with cognitive inflexibility in a rat model of schizophrenia. Nat Commun 11:2329 (2020). PubMed: 32393757