Anti-Myelin Basic Protein 抗体 [12] (ab7349)
![Western blot - Anti-Myelin Basic Protein antibody [12] (ab7349)](https://www.abcam.co.jp/ps/products/7/ab7349/Images/ab7349-12904-anti-myelin-basic-protein-antibody-12-western-blot.gif "Western blot - Anti-Myelin Basic Protein antibody [12] (ab7349)")
Key features and details
- Rat monoclonal [12] to Myelin Basic Protein
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG2a
製品の概要
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製品名
Anti-Myelin Basic Protein antibody [12]
Myelin Basic Protein 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [12] to Myelin Basic Protein -
由来種
Rat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Cow
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免疫原
Full length protein corresponding to Cow Myelin Basic Protein.
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エピトープ
Amino acids 82-87 (DENPVV). -
ポジティブ･コントロール
- WB: Mouse brain tissue lysate. IHC-P: Mouse cerebral cortex tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.1% Sodium azide
Constituent: Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
12 -
ミエローマ
NS0 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7349の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. |
ターゲット情報
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機能
The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. -
組織特異性
MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. -
関連疾患
Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease). -
配列類似性
Belongs to the myelin basic protein family. -
発生段階
Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter. -
翻訳後修飾
Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated. -
細胞内局在
Myelin membrane. Cytoplasmic side of myelin. - Information by UniProt
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参照データベース
- Entrez Gene: 618684 Cow
- Entrez Gene: 17196 Mouse
- SwissProt: P02687 Cow
- SwissProt: P04370 Mouse
- Unigene: 252063 Mouse
- Unigene: 392350 Mouse
- Unigene: 454459 Mouse
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別名
- GDB antibody
- Golli MBP antibody
- Golli MBP; myelin basic protein antibody
see all
画像
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Western blot - Anti-Myelin Basic Protein antibody [12] (ab7349)Anti-Myelin Basic Protein antibody [12] (ab7349) + Mouse brain tissue lysate
Observed band size: 19,26 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (170)
ab7349 は 170 報の論文で使用されています。
- Wu S et al. Retinoic acid increases phagocytosis of myelin by macrophages. J Cell Physiol 236:3929-3945 (2021). PubMed: 33165955
- Pu A et al. The glycosyltransferase EXTL2 promotes proteoglycan deposition and injurious neuroinflammation following demyelination. J Neuroinflammation 17:220 (2020). PubMed: 32703234
- Lee BY & Hur EM A Role of Microtubules in Oligodendrocyte Differentiation. Int J Mol Sci 21:N/A (2020). PubMed: 32033476
- Kawamura A et al. Oligodendrocyte dysfunction due to Chd8 mutation gives rise to behavioral deficits in mice. Hum Mol Genet 29:1274-1291 (2020). PubMed: 32142125
- You HJ et al. Mesenchymal stem cell-derived exosomes improve motor function and attenuate neuropathology in a mouse model of Machado-Joseph disease. Stem Cell Res Ther 11:222 (2020). PubMed: 32513306