製品の概要

  • 製品名

  • 製品の詳細

    Rabbit polyclonal to MyD88
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WBmore details
  • 種交差性

    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human MyD88 aa 279-296 (C terminal).
    Sequence:

    CTKSWFWTRLAKALSLP


    (Peptide available as ab7878)

  • ポジティブ・コントロール

    • Jurkat whole cell lysate (ab7899).
  • 特記事項

    MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 33 kDa).

ターゲット情報

  • 機能

    Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway.
  • 組織特異性

    Ubiquitous.
  • 関連疾患

    Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age.
  • 配列類似性

    Contains 1 death domain.
    Contains 1 TIR domain.
  • ドメイン

    The intermediate domain (ID) is required for the phosphorylation and activation of IRAK.
  • 細胞内局在

    Cytoplasm.
  • Information by UniProt
  • 参照データベース

  • 別名

    • Mutant myeloid differentiation primary response 88 antibody
    • MYD 88 antibody
    • Myd88 antibody
    • MYD88_HUMAN antibody
    • MYD88D antibody
    • Myeloid differentiation marker 88 antibody
    • Myeloid differentiation primary response 88 antibody
    • Myeloid differentiation primary response gene (88) antibody
    • Myeloid differentiation primary response gene 88 antibody
    • Myeloid differentiation primary response gene antibody
    • Myeloid differentiation primary response protein MyD88 antibody
    • OTTHUMP00000161718 antibody
    • OTTHUMP00000208595 antibody
    • OTTHUMP00000209058 antibody
    • OTTHUMP00000209059 antibody
    • OTTHUMP00000209060 antibody
    see all

画像

  • All lanes : Anti-MyD88 antibody (ab2064) at 1 µg/ml

    Lanes 1-2 : Wild type MEFs whole cell lysate
    Lanes 3-4 : MyD88 knockout MEFs whole cell lysate

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 33 kDa
    Observed band size: 33 kDa


    Exposure time: 15 seconds

    See Abreview

  • Anti-MyD88 antibody (ab2064) at 1/500 dilution + Jurkat Whole Cell Lysate

    Predicted band size: 33 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?

プロトコール

参考文献

This product has been referenced in:

  • DE Moura CFG  et al. Evaluation of the Chemopreventive Activity of Grape Skin Extract Using Medium-term Oral Carcinogenesis Assay Induced by 4-Nitroquinoline 1-Oxide. Anticancer Res 39:177-182 (2019). Read more (PubMed: 30591456) »
  • Cai J  et al. Recombinant fimbriae protein of Porphyromonas gingivalis induces an inflammatory response via the TLR4/NF-?B signaling pathway in human peripheral blood mononuclear cells. Int J Mol Med 43:1430-1440 (2019). Read more (PubMed: 30664173) »
See all 61 Publications for this product

レビューと Q&A

1-10 of 23 Abreviews or Q&A

Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Mouse Cell lysate - whole cell (MEFs)
Specification
MEFs
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

投稿 Feb 02 2015

Application
Western blot
Sample
Mouse Cell lysate - whole cell (macrophages cell line)
Loading amount
50 µg
Specification
macrophages cell line
Treatment
LPS
Gel Running Conditions
Reduced Denaturing (10-12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Varkha Agrawal

Verified customer

投稿 Sep 10 2012

Answer

Thank you for your reply. I will be happy to mail the datasheets to you. Are you having trouble accessing the datasheets online? I only ask so that I can inform our IT department if there are problems. Could you also please send me the address that you would like the datasheets sent to. Thank you and I look forward to your reply.

Read More

Answer

The datasheets are accessible from the product pages on the abcam.com website. You could try and print them from there. If you still have difficulty, please let me know and I will mail the datasheets to you.

Read More

Answer

Thank you for contacting Abcam. Please find attached the missing datasheets. If there is anything else I can help you with, please let me kn

Read More

Question
Answer

Thank you for contacting us. I am sorry that the vial you received did not contain the full amount of the product and I apologize for the inconvenience. I have issued a free of charge replacement vial which you will receive tomorrow. Please do not hesitate to contact us if you need anything further.

Read More

Question

Thank you for your email.I understand that GR 4851-3 & GR 4851-4 antibodies are from the same production batch, but then, I cannot understand why the two aliquots of antibodies gave different specificity results. I attached two pictures from Western blots done with liver samples from mice (20 ug).  Conditions are exactly the same,  in fact the first aliquot of antibody GR 4851-3, was used to establish those conditions in our lab (see below).  However, when we used a second aliquot of the same antibody (GR 4851-4) with the same liver samples that were used before, the results were quite disappointing. We observed many unspecific binging of the primary antibody, which did not diminished with higher dilutions (from 1:250 to 1:500) of the antibody or with longer blocking time (from 1 hour to 2hours) with BSA 2.5% . Western Blot Condition established with the GR 4851-3 aliquot:·       Blocking in BSA 2.5% or 5% in TTBS 1% for 1 hour or 2 hours (Blocking in Milk was also tried)·       Washing 3-4 times for 5 min in TTBS 1%·       1° antibody: different dilution factors have been tested and the one that gave better results was the 1:250 in 10 ml BSA 2.5%. Overnight incubation.·       2° antibody in BSA 2.5% TTBS 1% (unspecific binding given by the 2° antibody alone were not detected) Now that we received another GR 4851-4 aliquot, what are we supposed to do?If we test this new aliquot with the same conditions and with the same samples and have not good results, are we then entitled to be refunded? If this new aliquot works well, will we be refunded for the one that did not give good results? How will we know if further aliquots will be good or not? Considering the high amount of antibody which is required for each WB procedure (about 40 ul) and the total amount of antibody contained in each aliquot 100ul, you understand that we do not have the chance to waste antibody to test if the aliquot is a good one or not. Looking forward to hear from you soon.  

Read More
Answer

Thank you for your reply. I am sorry that you did not have a good experience with a previous vial of ab2064 lot GR 4851-4. I would like to reassure you that if you use this new antibody and do not obtain the desired results, I would be happy to replace this antibody free of charge. I encourage you to test this new vial of ab2064. However, please let me know if the new vial works for you. Also, can you provide an order number for the previous vial of ab2064 you purchased that did not work? I wish you best of luck with your experiments. Please do not hesitate to contact us if you have any additional questions.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human Normal Tonsil)
Specification
Human Normal Tonsil
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: DAKO Flex High pH-PTLink
Permeabilization
No
Blocking step
(agent) for 5 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

投稿 May 27 2011

Application
Western blot
Sample
Rat Tissue lysate - whole (liver)
Loading amount
20 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

投稿 Aug 17 2010

Application
Western blot
Sample
Rat Cell lysate - whole cell (smooth muscle cells)
Loading amount
15 µg
Specification
smooth muscle cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

投稿 Apr 21 2009

1-10 of 23 Abreviews or Q&A

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