Anti-MyD88 抗体 [4D6] (ab107585)
Key features and details
- Mouse monoclonal [4D6] to MyD88
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-MyD88 antibody [4D6]
MyD88 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [4D6] to MyD88 -
由来種
Mouse -
特異性
Espected to recognise Isoforms 1, 3, 4, 5, CRAa and CRAb. -
アプリケーション
適用あり: Flow Cyt (Intra), WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide, corresponding to amino acids 50-100 of Human MyD88 (NP_002459).
-
ポジティブ・コントロール
- human ovary, human prostate and Jurkat cell lysates and CF-7 cells. Flow Cyt (Intra): Jurkat cells
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
-
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
4D6 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab107585の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
Use 0.5µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
WB |
Use a concentration of 5 - 7 µg/ml. Predicted molecular weight: 33 kDa.
|
|
ICC/IF |
Use a concentration of 5 µg/ml.
|
特記事項 |
---|
Flow Cyt (Intra)
Use 0.5µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
Use a concentration of 5 - 7 µg/ml. Predicted molecular weight: 33 kDa. |
ICC/IF
Use a concentration of 5 µg/ml. |
ターゲット情報
-
機能
Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. -
組織特異性
Ubiquitous. -
関連疾患
Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age. -
配列類似性
Contains 1 death domain.
Contains 1 TIR domain. -
ドメイン
The intermediate domain (ID) is required for the phosphorylation and activation of IRAK. -
細胞内局在
Cytoplasm. - Information by UniProt
-
参照データベース
- Entrez Gene: 4615 Human
- Omim: 602170 Human
- SwissProt: Q99836 Human
- Unigene: 82116 Human
-
別名
- Mutant myeloid differentiation primary response 88 antibody
- MYD 88 antibody
- Myd88 antibody
see all
画像
-
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: MyD88 knockout HAP1 cell lysate (20 µg)
Lane 3: Jurkat cell lysate (20 µg)
Lane 4: MOLT4 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab107585 observed at 37 kDa. Red - loading control, ab176560, observed at 52 kDa.
ab107585 was shown to recognize MyD88 when MyD88 knockout samples were used, along with additional cross-reactive bands. Wild-type and MyD88 knockout samples were subjected to SDS-PAGE. ab107585 and ab176560 (loading control to alpha tubulin) were diluted 5 µg/mL and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
Intracellular flow cytometric analysis of MyD88 antibody (ab107585) in Jurkat cells (0.5 ug). Shaded histogram represents cells without antibody; green represents isotype control; red represents anti-MyD88 antibody. Secondary antibody- anti-mouse IgG PE conjugated.
-
Immunocytochemical analysis of MyD88 antibody (ab107585) in MCF-7 cells using an isotype control (top) and Myd88 Antibody (5 µg/ml; bottom).
-
All lanes : Anti-MyD88 antibody [4D6] (ab107585) at 5 µg/ml
Lane 1 : Human ovary cell lysate
Lane 2 : Human prostate cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Jurkat cell lysate with Immunizing peptide
Secondary
All lanes : Goat anti-mouse Ig HRP
Predicted band size: 33 kDa
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (3)
ab107585 は 3 報の論文で使用されています。
- Liu W et al. Heparin alleviates LPS-induced endothelial injury by regulating the TLR4/MyD88 signaling pathway. Exp Ther Med 22:1397 (2021). PubMed: 34650645
- Liu TY et al. Polygonatum sibiricum polysaccharides prevent LPS-induced acute lung injury by inhibiting inflammation via the TLR4/Myd88/NF-?B pathway. Exp Ther Med 20:3733-3739 (2020). PubMed: 32855724
- Sun Y et al. Exercise intervention alleviates nerve injury by the suppression of inflammatory mediator expression via the TLR4/NF-?B signaling pathway. Exp Ther Med 16:2922-2930 (2018). PubMed: 30233666