遺伝子ノックアウト細胞株 検証済

Anti-MyD88 抗体 [4D6] (ab107585)

Abreviews (1)Q&A (1)

製品の概要

  • 製品名
    Anti-MyD88 antibody [4D6]
    MyD88 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [4D6] to MyD88
  • 由来種
    Mouse
  • 特異性
    Espected to recognise Isoforms 1, 3, 4, 5, CRAa and CRAb.
  • アプリケーション
    適用あり: WB, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 50-100 of Human MyD88 (NP_002459).

  • ポジティブ･コントロール
    • human ovary, human prostate and Jurkat cell lysates and CF-7 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab107585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 5 - 7 µg/ml. Predicted molecular weight: 33 kDa.
Flow Cyt Use 0.5µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway.
  • 組織特異性
    Ubiquitous.
  • 関連疾患
    Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age.
  • 配列類似性
    Contains 1 death domain.
    Contains 1 TIR domain.
  • ドメイン
    The intermediate domain (ID) is required for the phosphorylation and activation of IRAK.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
    • 別名
      • Mutant myeloid differentiation primary response 88 antibody
      • MYD 88 antibody
      • Myd88 antibody
      • MYD88_HUMAN antibody
      • MYD88D antibody
      • Myeloid differentiation marker 88 antibody
      • Myeloid differentiation primary response 88 antibody
      • Myeloid differentiation primary response gene (88) antibody
      • Myeloid differentiation primary response gene 88 antibody
      • Myeloid differentiation primary response gene antibody
      • Myeloid differentiation primary response protein MyD88 antibody
      • OTTHUMP00000161718 antibody
      • OTTHUMP00000208595 antibody
      • OTTHUMP00000209058 antibody
      • OTTHUMP00000209059 antibody
      • OTTHUMP00000209060 antibody
      see all

    画像

    • Western blot - Anti-MyD88 antibody [4D6] (ab107585)
      Western blot - Anti-MyD88 antibody [4D6] (ab107585)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: MyD88 knockout HAP1 cell lysate (20 µg)
      Lane 3: Jurkat cell lysate (20 µg)
      Lane 4: MOLT4 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab107585 observed at 37 kDa. Red - loading control, ab176560, observed at 52 kDa.
      ab107585 was shown to recognize MyD88 when MyD88 knockout samples were used, along with additional cross-reactive bands. Wild-type and MyD88 knockout samples were subjected to SDS-PAGE. ab107585 and ab176560 (loading control to alpha tubulin) were diluted 5 µg/mL and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Flow Cytometry - Anti-MyD88 antibody [4D6] (ab107585)
      Flow Cytometry - Anti-MyD88 antibody [4D6] (ab107585)
      Intracellular flow cytometric analysis of MyD88 antibody (ab107585) in Jurkat cells (0.5 ug). Shaded histogram represents cells without antibody; green represents isotype control; red represents anti-MyD88 antibody. Secondary antibody- anti-mouse IgG PE conjugated.
    • Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody [4D6] (ab107585)
      Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody [4D6] (ab107585)
      Immunocytochemical analysis of MyD88 antibody (ab107585) in MCF-7 cells using an isotype control (top) and Myd88 Antibody (5 µg/ml; bottom).
    • Western blot - Anti-MyD88 antibody [4D6] (ab107585)
      Western blot - Anti-MyD88 antibody [4D6] (ab107585)
      All lanes : Anti-MyD88 antibody [4D6] (ab107585) at 5 µg/ml

      Lane 1 : Human ovary cell lysate
      Lane 2 : Human prostate cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : Jurkat cell lysate with Immunizing peptide

      Secondary
      All lanes : Goat anti-mouse Ig HRP

      Predicted band size: 33 kDa

    参考文献

    ab107585 has not yet been referenced specifically in any publications.

    ab107585 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

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    Western blot abreview for Anti-MyD88 antibody [4D6]

     Inconclusive
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (B-cells)
    Loading amount
    30 µg
    Specification
    B-cells
    Treatment
    BL19LP overexpress MyD88
    Gel Running Conditions
    Reduced Denaturing (10% SDS-Acrylamide gel 150V, 1h)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    投稿 Feb 08 2013

    ab107585, Abtrial: Maus

    DISCOUNT CODE: ABTRIAL-0000
    Ablaufdatum: 01 März. 2012

    Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab107585 in Maus zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Guts...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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