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遺伝子ノックアウト細胞株 検証済

Anti-MyD88 抗体 [4D6] (ab107585)

Reviews (1)Q&A (1)References (2)
Western blot - Anti-MyD88 antibody [4D6] (ab107585)
  • Flow Cytometry (Intracellular) - Anti-MyD88 antibody [4D6] (ab107585)
  • Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody [4D6] (ab107585)
  • Western blot - Anti-MyD88 antibody [4D6] (ab107585)

Key features and details

  • Mouse monoclonal [4D6] to MyD88
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG1

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-MyD88 antibody [EPR590(N)] (ab133739)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-MyD88 antibody [4D6]
    MyD88 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [4D6] to MyD88

  • 由来種

    Mouse

  • 特異性

    Espected to recognise Isoforms 1, 3, 4, 5, CRAa and CRAb.

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, ICC/IFmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide, corresponding to amino acids 50-100 of Human MyD88 (NP_002459).

  • ポジティブ･コントロール

    • human ovary, human prostate and Jurkat cell lysates and CF-7 cells. Flow Cyt (Intra): Jurkat cells

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab107585の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
Use 0.5µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB
Use a concentration of 5 - 7 µg/ml. Predicted molecular weight: 33 kDa.
ICC/IF
Use a concentration of 5 µg/ml.
特記事項
Flow Cyt (Intra)
Use 0.5µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB
Use a concentration of 5 - 7 µg/ml. Predicted molecular weight: 33 kDa.
ICC/IF
Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能

    Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway.

  • 組織特異性

    Ubiquitous.

  • 関連疾患

    Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age.

  • 配列類似性

    Contains 1 death domain.
    Contains 1 TIR domain.

  • ドメイン

    The intermediate domain (ID) is required for the phosphorylation and activation of IRAK.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Mutant myeloid differentiation primary response 88 antibody
      • MYD 88 antibody
      • Myd88 antibody
      • MYD88_HUMAN antibody
      • MYD88D antibody
      • Myeloid differentiation marker 88 antibody
      • Myeloid differentiation primary response 88 antibody
      • Myeloid differentiation primary response gene (88) antibody
      • Myeloid differentiation primary response gene 88 antibody
      • Myeloid differentiation primary response gene antibody
      • Myeloid differentiation primary response protein MyD88 antibody
      • OTTHUMP00000161718 antibody
      • OTTHUMP00000208595 antibody
      • OTTHUMP00000209058 antibody
      • OTTHUMP00000209059 antibody
      • OTTHUMP00000209060 antibody
      see all

    画像

    • Western blot - Anti-MyD88 antibody [4D6] (ab107585)
      Western blot - Anti-MyD88 antibody [4D6] (ab107585)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: MyD88 knockout HAP1 cell lysate (20 µg)
      Lane 3: Jurkat cell lysate (20 µg)
      Lane 4: MOLT4 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab107585 observed at 37 kDa. Red - loading control, ab176560, observed at 52 kDa.
      ab107585 was shown to recognize MyD88 when MyD88 knockout samples were used, along with additional cross-reactive bands. Wild-type and MyD88 knockout samples were subjected to SDS-PAGE. ab107585 and ab176560 (loading control to alpha tubulin) were diluted 5 µg/mL and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Flow Cytometry (Intracellular) - Anti-MyD88 antibody [4D6] (ab107585)
      Flow Cytometry (Intracellular) - Anti-MyD88 antibody [4D6] (ab107585)
      Intracellular flow cytometric analysis of MyD88 antibody (ab107585) in Jurkat cells (0.5 ug). Shaded histogram represents cells without antibody; green represents isotype control; red represents anti-MyD88 antibody. Secondary antibody- anti-mouse IgG PE conjugated.
    • Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody [4D6] (ab107585)
      Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody [4D6] (ab107585)
      Immunocytochemical analysis of MyD88 antibody (ab107585) in MCF-7 cells using an isotype control (top) and Myd88 Antibody (5 µg/ml; bottom).
    • Western blot - Anti-MyD88 antibody [4D6] (ab107585)
      Western blot - Anti-MyD88 antibody [4D6] (ab107585)
      All lanes : Anti-MyD88 antibody [4D6] (ab107585) at 5 µg/ml

      Lane 1 : Human ovary cell lysate
      Lane 2 : Human prostate cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : Jurkat cell lysate with Immunizing peptide

      Secondary
      All lanes : Goat anti-mouse Ig HRP

      Predicted band size: 33 kDa

    参考文献 (2)

    ab107585 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab107585 は 2 報の論文で使用されています。

    • Liu TY  et al. Polygonatum sibiricum polysaccharides prevent LPS-induced acute lung injury by inhibiting inflammation via the TLR4/Myd88/NF-?B pathway. Exp Ther Med 20:3733-3739 (2020). PubMed: 32855724
    • Sun Y  et al. Exercise intervention alleviates nerve injury by the suppression of inflammatory mediator expression via the TLR4/NF-?B signaling pathway. Exp Ther Med 16:2922-2930 (2018). PubMed: 30233666

    レビューと Q&A

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    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-MyD88 antibody [4D6]

     Inconclusive
    Abreviews
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (B-cells)
    Loading amount
    30 µg
    Specification
    B-cells
    Treatment
    BL19LP overexpress MyD88
    Gel Running Conditions
    Reduced Denaturing (10% SDS-Acrylamide gel 150V, 1h)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Feb 08 2013

    Question

    ab107585, Abtrial: Maus

    Read More
    Answer

    DISCOUNT CODE: ABTRIAL-0000
    Ablaufdatum: 01 März. 2012

    Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab107585 in Maus zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetzen, sobald Sie uns ein Abreview für diesen Antikörper eingereicht haben. Der Discountcode hat die Nummer ABTRIAL-0000, deckt den Wert von ab107585 ab und kann zum Kauf weiterer Produkte eingesetzt werden.

    Der Code wird gültig, sobald Sie uns ein Abreview für diesen Antikörper über den Test in Maus eingereicht haben. Bitte geben Sie diesen Code auch im Abschnitt “Additional Notes” des Abreviews mit an, so dass wir wissen, dass sich dieses Abreview auf die Gutscheinaktion bezieht. Der Code wird dadurch aktiviert. Bitte kontaktieren Sie bei der nächsten Bestellung unseren Kundendienst mit dem Code und der ursprünglichen Bestellnummer.

    Bitte zögern Sie nicht, sich wieder an uns zu wenden, falls Sie weitere Fragen haben. Wir freuen uns auf Ihr Abreview, egal ob Ihre Ergebnisse positiv oder negativ sind und wünschen Ihnen viel Glück für Ihre Forschung.

    Die Bedingungen dieses Angebotes befinden sich unter dem folgenden Link: www.abcam.com/collaborationdiscount

    Read More

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