Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lungs)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRS PH.9
Specification
Lungs
Blocking step
Peroxidase block for 15 min followed by Serum free protein block for 12 min as blocking agent for 15 minute(s) · Concentration: 0% · Temperature: RT°C
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s)
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: EnVision – labelled polymer HRP anti-rabbit
Host species: Rabbit
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Rabbit
Clonality: Polyclonal
Conjugation: HRP polymer
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Syeda Mariam Riaz
Verified customer
投稿 Oct 14 2020