Biotin Anti-Myc tag 抗体 [Myc.A7] (ab173829)
Key features and details
- Biotin Mouse monoclonal [Myc.A7] to Myc tag
- Suitable for: ICC/IF, IP, ELISA, WB
- Conjugation: Biotin
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Biotin Anti-Myc tag antibody [Myc.A7]
Myc tag 一次抗体 製品一覧 -
製品の詳細
Biotin Mouse monoclonal [Myc.A7] to Myc tag -
由来種
Mouse -
標識
Biotin -
アプリケーション
適用あり: ICC/IF, IP, ELISA, WBmore details -
免疫原
Synthetic peptide corresponding to Human Myc tag aa 410-419 (C terminal).
Sequence:EQKLISEEDL
Database link: P01106 -
ポジティブ・コントロール
- E. coli lysate containing a multi-epitope tagged protein.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
Myc.A7 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab173829の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/500 - 1/2000.
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IP |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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WB |
1/500 - 1/1000. Predicted molecular weight: 1.2 kDa.
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特記事項 |
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ICC/IF
1/500 - 1/2000. |
IP
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Predicted molecular weight: 1.2 kDa. |
ターゲット情報
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関連性
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. -
細胞内局在
Nuclear -
別名
- c-myc tag antibody
- Myc Epitope Tag antibody
画像
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All lanes : Biotin Anti-Myc tag antibody [Myc.A7] (ab173829) at 1/1000 dilution
Lane 1 : E. coli lysate containing a multi-epitope tagged protein at 0.25 µg
Lane 2 : E. coli lysate containing a multi-epitope tagged protein at 0.125 µg
Lane 3 : E. coli lysate containing a multi-epitope tagged protein at 0.0599999986588955 µg
Lane 4 : E. coli lysate containing a multi-epitope tagged protein at 0.0299999993294477 µg
Lane 5 : E. coli lysate containing a multi-epitope tagged protein at 0.0149999996647239 µg
Secondary
All lanes : Streptavidin-HRP at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 1.2 kDa
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab173829 は論文での使用が確認できていません。