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Anti-Myc tag 抗体 [Myc.A7] (ab18185)

Reviews (5)Q&A (2)References (35)
Western blot - Anti-Myc tag antibody [Myc.A7] (ab18185)
  • Western blot - Anti-Myc tag antibody [Myc.A7] (ab18185)

Key features and details

  • Mouse monoclonal [Myc.A7] to Myc tag
  • Suitable for: ELISA, Dot blot, WB, IP
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Biotin HRP

製品の概要

  • 製品名

    Anti-Myc tag antibody [Myc.A7]
    Myc tag 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [Myc.A7] to Myc tag

  • 由来種

    Mouse

  • 特異性

    Recognizes overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.

  • アプリケーション

    適用あり: ELISA, Dot blot, WB, IPmore details

  • 免疫原

    Synthetic peptide:

    EQKLISEEDL

    , corresponding to amino acids 410-419 of Human Myc.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • WB: HEK-293 whole cell lysate ICC/IF: rat primary hippocampal neurons

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 25th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • 特記事項（精製）

    Protein A affinity chromatography

  • ポリ/モノ

    モノクローナル

  • クローン名

    Myc.A7

  • アイソタイプ

    IgG1

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab18185の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
WB (1)
1/1000. Can be blocked with Human c-Myc peptide (ab13837).
IP
Use at an assay dependent concentration.
特記事項
ELISA
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
WB
1/1000. Can be blocked with Human c-Myc peptide (ab13837).
IP
Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

  • 細胞内局在

    Nuclear

  • 別名

    • c-myc tag antibody
    • Myc Epitope Tag antibody

画像

  • Western blot - Anti-Myc tag antibody [Myc.A7] (ab18185)
    Western blot - Anti-Myc tag antibody [Myc.A7] (ab18185)
    All lanes : Anti-Myc tag antibody [Myc.A7] (ab18185) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : MYC CRISPR-Cas9 edited HEK-293T cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : SH-SY5Y cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.


    False colour image of Western blot: Anti-Myc tag antibody [Myc.A7] staining at 1/1000 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab18185 was shown to bind specifically to Myc tag. A band was observed at 57 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in MYC CRISPR-Cas9 edited cell line ab256500 (CRISPR-Cas9 edited cell lysate ab263850). The band observed in the CRISPR-Cas9 edited lysate lane below 57 kDa is likely to represent a truncated form of Myc tag. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and MYC CRISPR-Cas9 edited HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

  • Western blot - Anti-Myc tag antibody [Myc.A7] (ab18185)
    Western blot - Anti-Myc tag antibody [Myc.A7] (ab18185)
    All lanes : Anti-Myc tag antibody [Myc.A7] (ab18185) at 1/1000 dilution

    Lane 1 : HEK293 lysate - untransfected
    Lane 2 : HEK293 lysate - transfected with Myc-tagged protein

参考文献 (35)

ab18185 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab18185 は 35 報の論文で使用されています。

  • Castro-Gonzalez S  et al. HIV-1 Nef counteracts autophagy restriction by enhancing the association between BECN1 and its inhibitor BCL2 in a PRKN-dependent manner. Autophagy 17:553-577 (2021). PubMed: 32097085
  • Dong HL  et al. Biallelic SORD pathogenic variants cause Chinese patients with distal hereditary motor neuropathy. NPJ Genom Med 6:1 (2021). PubMed: 33397963
  • Wang D  et al. FKBP25 Regulates Meiotic Apparatus During Mouse Oocyte Maturation. Front Cell Dev Biol 9:625805 (2021). PubMed: 33553183
  • Stavsky A  et al. Aberrant activity of mitochondrial NCLX is linked to impaired synaptic transmission and is associated with mental retardation. Commun Biol 4:666 (2021). PubMed: 34079053
  • Na HJ  et al. Nutrient-Driven O-GlcNAcylation Controls DNA Damage Repair Signaling and Stem/Progenitor Cell Homeostasis. Cell Rep 31:107632 (2020). PubMed: 32402277
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-7 of 7 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Myc tag antibody [Myc.A7]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293T plus Rab5-myc)
Permeabilization
Yes - Triton-X 100
Specification
HEK293T plus Rab5-myc
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 May 28 2021

Immunocytochemistry abreview for Anti-Myc tag antibody [Myc.A7]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cell (Melanoma cells transfected with gp100-myc)
Permeabilization
Yes - .1% TritonX-100
Specification
Melanoma cells transfected with gp100-myc
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Sep 03 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-Myc tag antibody [Myc.A7]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293T)
Specification
HEK293T
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Acetone
Read More

Abcam user community

Verified customer

投稿 Jun 05 2020

Western blot abreview for Anti-Myc tag antibody [Myc.A7]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293T + SPPL3-myc)
Gel Running Conditions
Reduced Denaturing
Loading amount
200000 cells
Specification
HEK 293T + SPPL3-myc
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

HERR DR. Christian Ostalecki

Verified customer

投稿 Jul 20 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-Myc tag antibody [Myc.A7]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Primary hippocampal neurons)
Specification
Primary hippocampal neurons
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 20°C
Read More

Abcam user community

Verified customer

投稿 Mar 11 2010

Question

Hello there
I have purchased monoclonal Myc Ab.
https://www.abcam.com/Myc-tag-antibody-Myc-A7-ab18185.html
I would like to know what is the Kd for this antibody against the Myc
epitope.It would be quite helpful for me.
Regards

Read More
Answer

Thank you for your enquiry,

I am sorry to confirm that the Kd for ab18185 Myc tag antibody [Myc.A7] has not been assessed. It is unusual for the Kd to be measured for research grade antibodies.

We aim to provide as much information as possible to customers, and I am sorry we do not have the details you require on this occasion. If you have any further questions, please do not hesitate to contact us.

Read More

Question

Heu there
Thanks for the reply
We have selected some peptide aptamers against Myc antibodies.we have
the myc epitopes in the loops of the peptide aptamers.the selection
was done using polyclonal antibodies.It was an ELISA detection.Now we
would like to shift to a monoclonal Ab because we think that using
monoclonal Ab would be more specific and more appropriate for
comparing the dissociation constants of the different binders.
Regards

Read More
Answer

Thank you for getting back to me and for providing some further information about your research project.

Currently, we have 2 monoclonal antibodies against Myc tag: ab18185 and ab94936. Although they are from the same clone but from two sources and the testing conditions were different. ab94936 reacts with recombinant fragment so the other antibody ab18185 may suit your need better.

There are two specific publication for ab18185 you could take a look at to get some more information:

Biswas Ket al. A comprehensive functional characterization of BRCA2 variants associated with Fanconi anemia using mouse ES cell-based assay. Blood 118:2430-42 (2011). WB.http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=21719596&dopt=Abstract
Ohe Met al. Emi2 inhibition of the anaphase-promoting complex/cyclosome absolutely requires Emi2 binding via the C-terminal RL tail. Mol Biol Cell 21:905-13 (2010). WB.http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=20089832&dopt=Abstract



If you need any further assistance in the future, please do not hesitate to contact me.

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