製品の概要

  • 製品名

    Anti-Myc tag antibody (FITC)
    Myc tag 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Myc tag (FITC)
  • 由来種

    Rabbit
  • 標識

    FITC. Ex: 493nm, Em: 528nm
  • アプリケーション

    適用あり: ICC/IF, WB, ELISAmore details
  • 免疫原

    EQKLISEEDL (C-MYC) conjugated with KLH.

  • 特記事項

    Molar F/P ratio is 4.6:1

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C.
  • バッファー

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to fluorescein isothiocyanate.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab1393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/250. PubMed: 17443800
WB 1/1000 - 1/5000. Can be blocked with Human c-Myc peptide (ab13837).
ELISA 1/1000.

ターゲット情報

  • 関連性

    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • 細胞内局在

    Nuclear
  • 別名

    • c-myc tag antibody
    • Myc Epitope Tag antibody

参考文献

This product has been referenced in:

  • Xiao C  et al. Targeting HSP70 to motoneurons protects locomotor activity from hyperthermia in Drosophila. Dev Neurobiol 67:438-55 (2007). Read more (PubMed: 17443800) »
See 1 Publication for this product

レビューと Q&A

1-2 of 2 Abreviews or Q&A

Application
Flow Cytometry
Sample
Saccharomyces cerevisiae Cell (Yeast cells)
Permeabilization
No
Gating Strategy
Cells that were Myc-FITC positive
Specification
Yeast cells
Preparation
Cell harvesting/tissue preparation method: Harvested them in standard PBS with 0.1% BSA
Sample buffer: PBS with 0.1% BSA
Fixation
No fixiation

Dr. Kaushik Thakkar

Verified customer

投稿 Jul 03 2019

Answer

Thank you very much for your interest in ab1393.

Unfortunately we do not have more information about the use of the anti-Myc tag antibody (FITC) ab1393 in ICC/IF than the one available in the publication (PubMed: 17443800) and we do not currently have an image.

However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.

Simply follow these easy steps below to apply for our AbTrial Program:
1. Reply to this email, letting us know you are interested in testing this product.
2. Our scientists will email you an inactive personal discount code for the value of the product.
3. Purchase and test the product at the regular price.
4. Submit an image of your results, including your discount code in the additional notes section of your Abreview.
5. Once the Abreview is submitted, the discount code will become active.
6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/abtrial.

About the ICC/IF protocol, we recommend the following procedure: https://www.abcam.com/index.html?pageconfig=resource&rid=11417.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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