Anti-Myc tag 抗体 (ab9109)

Submit a review Q&A (3)References (2)

Key features and details

  • Chicken polyclonal to Myc tag
  • Suitable for: ELISA, WB
  • Isotype: IgY

製品の概要

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgY

  • 軽鎖の種類

    unknown

  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab9109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報
    ELISA: 1/200 - 1/500 (coating) and 1/5000 – 1/20000 (probing).
    WB: 1/5000.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • ターゲット情報

    • 関連性

      Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

    • 細胞内局在

      Nuclear

    • 別名

      • c-myc tag antibody
      • Myc Epitope Tag antibody

    参考文献 (2)

    ab9109 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9109 は 2 報の論文で使用されています。

    • Morii M  et al. Src Acts as an Effector for Ku70-dependent Suppression of Apoptosis through Phosphorylation of Ku70 at Tyr-530. J Biol Chem 292:1648-1665 (2017). PubMed: 27998981
    • Wang F  et al. Ca2+ homeostasis modulation enhances the amenability of L444P glucosylcerebrosidase to proteostasis regulation in patient-derived fibroblasts. ACS Chem Biol 6:158-68 (2011). ELISA . PubMed: 21043486

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    1-3 of 3 Abreviews or Q&A

    Question

    Inquiry: Hello, I am looking for a not-rabbit anti-Myc tag antibody to use in a double-staining procedure with another antibody made in rabbit. We have been successfully using the rabbit anti-Myc (Ab9106) antibody for immunostainings of Drosophila brain. Now, we would like to try the similar chicken anti-Myc (Ab9109) for a double-labeling but we are not sure if it works in Drosophila at all. I was wondering whether it is possible to obtain an aliquot of the Ab9109 antibody to try it in our assay before spending over $300 in the product. Once we know that it works in our system we will be happy to buy the commercially available package of that product.

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    Answer

    Thank you very much for contacting us with your question.

    Since the antibody ab9109 is specific for the Myc tag and not an endogenousDrosophila protein, we would guarantee this antibody to work in the tested applications of Western blot and ELISA with your samples. However, we have not tested ab9109 in any kind of immunostaining so we're not sure if it is suitable for this application. We don't provide sample aliquots of our products, but we do have a testing discount program that you are eligible for. As part of this program, you would need to first purchase ab9109 at full price, but after testing the antibody in immunostaining and submitting an online Abreview with your data and protocol, you would receive one free primary antibody on a future purchase.If you're interested in this program I can send you the full details.

    Wealso have several anti-chicken secondary antibodies in case you don't already have one. For an HRP conjugation, I would recommend ab7118, which has been tested in immunostaining and has been pre-adsorbed so that it won't cross-react with your other primary antibody-

    https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Chicken-IgY-HL-HRP-pre-adsorbed-ab7118.html

    For fluorescent detection, I would suggest ab96951 DyLight 488 conjugated, which has also been tested in immunostaining and is pre-adsorbed-

    https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Chicken-IgY-H-L-DyLight-488-pre-adsorbed-ab96951.html

    I hope that this information will be useful, but please let me know if you have any further questions or require further informaiton. I'll be happy to help. Have a nice day!

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    Question

    I'm sorry, I was wondering at wich concetration you would recommend it for immunofluorescence. thank's

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    Answer

    We do not have any data on the use of this antibody in immunofluorescence. However, if you are testing the antibody in this application, please let us know your results and in return we'll forward a EUR 25.00 Amazon gift voucher.

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    Question

    hello, i was wondering if you did check the immuno efficience of this ab ? (chicken anti c myc ab9109) thank's a lot Gautier

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    Answer

    I'm sorry, I don't quite understand your question. In WB, this antibody is reported to be able to detect the c-myc epitope tag at a 1:10,000 dilution.

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