Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
0.3% BSA/8%donkeyserum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 25°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (brain)
Specification
brain
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.3% BSA/0.33%TritonX-100/8%donkeyserum
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: alexa fluor donkey anti-rabbit 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Dilution
1/500
Additional data
Additional Notes
This is a brain electroplated at E14.5 with EGFP and a myc tagged protein, and then perfused at P28.Tissue then fixed with 4% PFA overnight, embedded in agar and sectioned on vibratome.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Karen Yee
Verified customer
投稿 Oct 02 2014