Application
Western blot
Sample
Tobacco Tissue lysate - whole (leaf)
Loading amount
20 µg
Specification
leaf
Gel Running Conditions
Non-reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Dilution
1/3000
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: ECL anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/8000
Detection
Detection method
ECL+
Exposure
15 second(s)
Positive control
FEA2 with myc
Negative control
non transfected
Additional data
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jan 28 2013