Anti-Myc tag 抗体 [9E11] - ChIP Grade (ab56)
製品の概要
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製品名
Anti-Myc tag antibody [9E11] - ChIP Grade
Myc tag 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [9E11] to Myc tag - ChIP Grade -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt, ChIP, WB, IP, IHC-P, IHC-Frmore details -
種交差性
交差種: Chicken, Human, Saccharomyces cerevisiae -
免疫原
Synthetic peptide corresponding to Human Myc tag aa 400-500 conjugated to keyhole limpet haemocyanin.
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ポジティブ・コントロール
- ウェスタン・ブロット用ポジティブコントロールRecombinant human c-Myc protein (Active)
- IP:CHO overexpressing Stra8 whole cell lysate. Flow Cyt: HL60 cells. WB: ab84132, ab5395 and HEK293 cell lysate
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特記事項
This antibody clone is manufactured by Abcam.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
モノクローナル -
クローン名
9E11 -
ミエローマ
Sp2 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab56 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt | 1/200. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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ChIP | Use at an assay dependent concentration. 2ul per 500ug of extract, see H. Zhang et al. | |
WB | 1/500 - 1/1000. Predicted molecular weight: 49 kDa. Additional non-specific bands observed at 75, 110, 140 kDa using mouse and human cells (see Abreview). | |
IP | Use a concentration of 5 µg/ml. | |
IHC-P | Use at an assay dependent concentration. | |
IHC-Fr | Use at an assay dependent concentration. |
ターゲット情報
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関連性
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 4609 Human
- Omim: 190080 Human
- SwissProt: P01106 Human
- Unigene: 202453 Human
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別名
- c-myc tag antibody
- Myc Epitope Tag antibody
画像
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Myc tag was immunoprecipitated using 0.5mg CHO overexpressing Stra8 whole cell lysate, 5µg of Mouse monoclonal to Myc tag and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, CHO overexpressing Stra8 whole cell lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab56.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.
Band: 49KDa; Myc tag
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Anti-Myc tag antibody [9E11] - ChIP Grade (ab56) at 1/500 dilution +
Recombinant Human c-Myc protein (ab84132) at 0.01 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Exposure time: 4 minutes -
Overlay histogram showing HL60 cells stained with ab56 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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All lanes : Anti-Myc tag antibody [9E11] - ChIP Grade (ab56) at 1 µg/ml
Lane 1 : HEK293 cell lysate at 20 µg
Lane 2 :Recombinant Human c-Myc protein (ab84132) at 0.1 µg
Lane 3 :E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.1 µg
Secondary
All lanes : Goat polyclonal to Mouse IgG H&L Pre-Adsorbed (HRP) at 1/5000 dilution
Predicted band size: 49 kDa
プロトコール
データシートおよび資料
参考文献
This product has been referenced in:
- Shang Z et al. LncRNA PCAT1 activates AKT and NF-?B signaling in castration-resistant prostate cancer by regulating the PHLPP/FKBP51/IKKa complex. Nucleic Acids Res N/A:N/A (2019). Read more (PubMed: 30773595) »
- Wong M et al. JMJD6 is a tumorigenic factor and therapeutic target in neuroblastoma. Nat Commun 10:3319 (2019). Read more (PubMed: 31346162) »