Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 抗体 [18C7.2] (ab77956)
Key features and details
- Mouse monoclonal [18C7.2] to Muscarinic Acetylcholine Receptor M4/CHRM4
- Suitable for: IHC-Fr, IP, Flow Cyt, WB
- Reacts with: Mouse, Rat, Human, Monkey
- Isotype: IgG1
製品の概要
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製品名
Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody [18C7.2]
Muscarinic Acetylcholine Receptor M4/CHRM4 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [18C7.2] to Muscarinic Acetylcholine Receptor M4/CHRM4 -
由来種
Mouse -
特異性
ab77956 reacts with Muscarinic Acetylcholine Receptor M4/CHRM4. There is no reactivity with the other subtypes.
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アプリケーション
適用あり: IHC-Fr, IP, Flow Cyt, WBmore details -
種交差性
交差種: Mouse, Rat, Human, Monkey -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Transfected CHO cell lines
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.1% Sodium azide
Constituents: 1.45% Sodium chloride, 0.0536% PBS -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Purified from TCS. -
ポリ/モノ
モノクローナル -
クローン名
18C7.2 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77956の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-Fr |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
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WB | (2) |
Use at an assay dependent concentration.
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特記事項 |
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IHC-Fr
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
ターゲット情報
- Information by UniProt
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参照データベース
- Entrez Gene: 1132 Human
- Entrez Gene: 12672 Mouse
- Entrez Gene: 25111 Rat
- Omim: 118495 Human
- SwissProt: P08173 Human
- SwissProt: P32211 Mouse
- SwissProt: P08485 Rat
- Unigene: 248100 Human
see all -
別名
- AChR antibody
- ACM4_HUMAN antibody
- Cholinergic receptor muscarinic 4 antibody
see all
画像
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Overlay histogram showing SH-SY5Y cells stained with ab77956 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77956, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab77956 は 5 報の論文で使用されています。
- Tarafder S et al. A Combination of Oxo-M and 4-PPBP as a potential regenerative therapeutics for tendon injury. Theranostics 9:4241-4254 (2019). PubMed: 31281545
- Thomson SR et al. Cell-Type-Specific Translation Profiling Reveals a Novel Strategy for Treating Fragile X Syndrome. Neuron 95:550-563.e5 (2017). PubMed: 28772121
- Mei F et al. Accelerated remyelination during inflammatory demyelination prevents axonal loss and improves functional recovery. Elife 5:N/A (2016). IHC-FoFr ; Mouse . PubMed: 27671734
- Zhao Z et al. Involvement of HCN Channel in Muscarinic Inhibitory Action on Tonic Firing of Dorsolateral Striatal Cholinergic Interneurons. Front Cell Neurosci 10:71 (2016). IHC . PubMed: 27047336
- Yokoi F et al. Decreased dopamine receptor 1 activity and impaired motor-skill transfer in Dyt1 ?GAG heterozygous knock-in mice. Behav Brain Res 279:202-10 (2015). PubMed: 25451552