Anti-Muscarinic Acetylcholine Receptor 2/CM2 抗体 [31-1D1] (ab2805)

Reviews (7)Q&A (6)References (12)

Key features and details

  • Mouse monoclonal [31-1D1] to Muscarinic Acetylcholine Receptor 2/CM2
  • Suitable for: WB, ICC, IHC-P
  • Reacts with: Mouse, Rat, Human, Pig
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1]
    Muscarinic Acetylcholine Receptor 2/CM2 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [31-1D1] to Muscarinic Acetylcholine Receptor 2/CM2

  • 由来種

    Mouse

  • 特異性

    This antibody is specific for the m2 mAChR subtype.

  • アプリケーション

    適用あり: WB, ICC, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Rat, Human, Pig
    非交差種: Chicken

  • 免疫原

    Full length native protein (purified) corresponding to Pig Muscarinic Acetylcholine Receptor 2/CM2. Purified from Pig Heart.

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 52 kDa).
ICC Use at an assay dependent concentration.
IHC-P 1/20.

ターゲット情報

  • 機能

    The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.

  • 関連疾患

    Genetic variations in CHRM2 can influence susceptibility to major depressive disorder (MDD) [MIM:608516]. MDD is one of the most common psychiatric disorders. MDD is a complex trait characterized by one or more major depressive episodes without a history of manic, mixed, or hypomanic episodes. A major depressive episode is characterized by at least 2 weeks during which there is a new onset or clear worsening of either depressed mood or loss of interest or pleasure in nearly all activities. Four additional symptoms must also be present including changes in appetite, weight, sleep, and psychomotor activity; decreased energy; feelings of worthlessness or guilt; difficulty thinking, concentrating, or making decisions; or recurrent thoughts of death or suicidal ideation, plans, or attempts. The episode must be accompanied by distress or impairment in social, occupational, or other important areas of functioning.

  • 配列類似性

    Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.

  • 細胞内局在

    Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 7TM receptor antibody
    • Acetylcholine receptor, muscarinic, 2 antibody
    • AChR antibody
    • Acm2 antibody
    • ACM2_HUMAN antibody
    • Cholinergic receptor muscarinic 2 antibody
    • Cholinergic receptor, muscarinic 2, cardiac antibody
    • Cholinergic receptor, muscarinic 2, isoform a antibody
    • Cholinergic receptor, muscarinic 2a antibody
    • CHRM 2 antibody
    • CHRM2 antibody
    • chrm2a antibody
    • CM2 antibody
    • FLJ43243 antibody
    • HM 2 antibody
    • HM2 antibody
    • M2 antibody
    • M2 muscarinic receptor antibody
    • M2-mAChR antibody
    • MGC120006 antibody
    • MGC120007 antibody
    • Muscarinic acetylcholine receptor M2 antibody
    • Muscarinic M2 receptor antibody
    see all

画像

  • Western blot - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1] (ab2805)
    Western blot - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1] (ab2805)
    All lanes : Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1] (ab2805) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human spinal cord tissue lysate - total protein (ab29188)
    Lane 3 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 52 kDa
    Observed band size: 64 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 22 kDa, 40 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds


    Muscarinic Acetylcholine Receptor 2/CM2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1] (ab2805)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1] (ab2805)

    Immunohistochemistry was performed on normal biopsies of deparaffinized human kidney tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer and microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Mouse monoclonal antibody recognizing Muscarinic Acetylcholine Receptor 2/CM2 (ab2805) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunocytochemistry - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1] (ab2805)
    Immunocytochemistry - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1] (ab2805)
    ICC/IF image of ab2805 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2805, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献 (12)

ab2805 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab2805 は 12 報の論文で使用されています。

  • Ma G  et al. Association of Autoantibodies against M2-Muscarinic Acetylcholine Receptor with Atrial Fibrosis in Atrial Fibrillation Patients. Cardiol Res Pract 2019:8271871 (2019). PubMed: 30863630
  • Leonhäuser D  et al. Expression of components of the urothelial cholinergic system in bladder and cultivated primary urothelial cells of the pig. BMC Urol 19:62 (2019). PubMed: 31288793
  • Tarafder S  et al. A Combination of Oxo-M and 4-PPBP as a potential regenerative therapeutics for tendon injury. Theranostics 9:4241-4254 (2019). PubMed: 31281545
  • Deng Y  et al. Angiotensin-Converting Enzyme 2 in the Rostral Ventrolateral Medulla Regulates Cholinergic Signaling and Cardiovascular and Sympathetic Responses in Hypertensive Rats. Neurosci Bull 35:67-78 (2019). PubMed: 30318562
  • Benoit A  et al. Flow cytometry for receptor analysis from ex-vivo brain tissue in adult rat. J Neurosci Methods 304:11-23 (2018). Flow Cyt ; Rat . PubMed: 29660368
View all Publications for this product

レビューと Q&A

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1-10 of 13 Abreviews or Q&A

Western blot abreview for Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1]

 Average
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
40 µg
Specification
brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

投稿 Nov 26 2020

Immunohistochemistry (Frozen sections) abreview for Anti-Muscarinic Acetylcholine Receptor 2 antibody [31-1D1]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (40 ´m skin sections)
Permeabilization
Yes - Triton X
Specification
40 ´m skin sections
Blocking step
BSA as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Mr. Christoph Erbacher

Verified customer

投稿 Aug 30 2017

Western blot abreview for Anti-Muscarinic Acetylcholine Receptor 2 antibody [31-1D1]

 Below Average
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing (4-12% BIS TRIS)
Loading amount
15 µg
Specification
Intestinal tissue
Blocking step
Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

投稿 Jul 13 2016

Western blot abreview for Anti-Muscarinic Acetylcholine Receptor 2 antibody [31-1D1]

 Below Average
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing
Loading amount
14 µg
Specification
Intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Mr. Julien Chevalier

Verified customer

投稿 Jul 08 2016

Western blot abreview for Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1]

 Inconclusive
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing
Loading amount
14 µg
Specification
Intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Jul 08 2016

Western blot abreview for Anti-Muscarinic Acetylcholine Receptor 2 antibody [31-1D1]

 Average
Abreviews
abreview image
Application
Western blot
Sample
Pig Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Jul 08 2016

Question

Customer kindly called as they were experiencing issue with these products.

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Answer

Thank you for contacting us. I hope that the suggestions which were made help you to resolve the issues which you have been experiencing. As requested, I have arranged for you to receive positive control lysates ab71719 and ab29330 free of charge. The confirmation number for these products is xxxxx. I have also created enrolled you into our 100% Abreview program for testing ab60981 in western blot. After submission of an Abreview the code xxxx will activate, entitling you to one free primary antibody of your choice. Further information about this program may be found at the following link:https://www.abcam.com/index.html?pageconfig=resource&rid=11998 I have also created for you a 25% off one item discount code which may be redeemed anytime before 17 January 2013. This 25% off code is xxxxx Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

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Question

Blocking peptide for ab2805

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Answer

Thank you for contacting us.

Unfortunately we do not have blocking peptides for any of the antibody ab2805, ab90805 and ab109226. I apologize for the inconvenience this may have caused.

I can however recommend trying the following protocol steps as alternative˜
- use negative control cells or tissue that does not express this protein. The human protein Atlas database can help in finding this.
- Use no primary control sections
- Use isotype control antibody

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Can you tell me if this product will also detect M1 or M3?

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Answer

Thank you for your enquiry. Unfortunately, we do not have the exact information you require but I was able to locate the following information in the system. 1) No cross-reaction with thodopsin which is highly homologous to mAChR by Western Blot. 2) Does not cross-react with M1 the neurological form of the receptor in IP (M1 and M2 are ~ 38% homologous). However, in checking the literature, the following article includes data that demonstrates the specificity of this antibody for M2 when tested against M1 - M4. P. Wang, G.R. Luthin and M.R. Ruggieri (1995). Muscarinic acetylcholine receptor subtypes mediating urinary bladder contractility and coupling to GTP binding proteins. J. Phamacol. and Exp. Therapeutics. 273 (2), 959 -273. I hope the above information will still be helpful.

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Question

I wish to use this antibody in tests with a mutant form of the protein. Where is the epitope of this antibody located?

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Answer

The monoclonal antibody to the Muscarinic 2 Acetylcholine Receptor has not been mapped, therefore the epitope could be contained anywhere on the protein. The immunogen is purified muscarinic receptor from pig heart. This product is specific for M2 Receptor. I hope this information helps. Please do not hesitate to contact us if you need anything further.

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1-10 of 13 Abreviews or Q&A

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