Anti-Musashi 1 / Msi1 抗体 (ab21628)
Key features and details
- Rabbit polyclonal to Musashi 1 / Msi1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Musashi 1 / Msi1 antibody
Musashi 1 / Msi1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Musashi 1 / Msi1 -
由来種
Rabbit -
特異性
Several customers have found that this antibody gives good results in rat however in our hands, we cannot obtain positive results. This antibody is therefore no longer covered by our Abpromise guarantee for use in rat. -
アプリケーション
適用あり: WBmore details
適用なし: ICC or IHC-P -
種交差性
交差種: Mouse, Rat, Human -
免疫原
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ポジティブ･コントロール
- IHC: Human small intestine, and hippocampus tissues
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Coimmunogen
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab21628の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
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| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). |
ターゲット情報
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機能
RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system. -
組織特異性
Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines. -
配列類似性
Belongs to the Musashi family.
Contains 2 RRM (RNA recognition motif) domains. -
ドメイン
The first RNA recognition motif binds more strongly to RNA compared to the second one. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 4440 Human
- Entrez Gene: 17690 Mouse
- Entrez Gene: 259272 Rat
- Omim: 603328 Human
- SwissProt: O43347 Human
- SwissProt: Q61474 Mouse
- SwissProt: Q8K3P4 Rat
- Unigene: 158311 Human
see all -
別名
- Msi 1 antibody
- Msi1 antibody
- MSI1H_HUMAN antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody (ab21628)
IHC image of Musashi 1 / Msi1 staining in a section of formalin-fixed paraffin-embedded normal human hippocampus* performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab21628, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody (ab21628)IHC image of Musashi 1 / Msi1 staining in a section of formalin-fixed paraffin-embedded normal human small intestine* performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab21628, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-Musashi 1 / Msi1 antibody (ab21628)All lanes : Anti-Musashi 1 / Msi1 antibody (ab21628) at 1 µg/ml
Lane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 2 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205)
Lane 3 : E14 Mouse Embryo Brain Tissue Lysate
Lane 4 : E10 Mouse Embryo Brain Tissue Lysate
Lane 5 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate with Human Musashi 1 / Msi1 peptide (ab23870) at 1 µg/ml
Lane 6 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205) with Human Musashi 1 / Msi1 peptide (ab23870) at 1 µg/ml
Lane 7 : E14 Mouse Embryo Brain Tissue Lysate with Human Musashi 1 / Msi1 peptide (ab23870) at 1 µg/ml
Lane 8 : E10 Mouse Embryo Brain Tissue Lysate with Human Musashi 1 / Msi1 peptide (ab23870) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.
ab21628 recognized a band corresponding to the expected size of Musashi1/Msi1. The band was blocked using the immunising peptide (ab23870).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (30)
ab21628 は 30 報の論文で使用されています。
- Allensworth-James M et al. Control of the Anterior Pituitary Cell Lineage Regulator POU1F1 by the Stem Cell Determinant Musashi. Endocrinology 162:N/A (2021). PubMed: 33373440
- Jia J et al. Transcriptional factor FoxM1-activated microRNA-335-3p maintains the self-renewal of neural stem cells by inhibiting p53 signaling pathway via Fmr1. Stem Cell Res Ther 12:169 (2021). PubMed: 33691791
- Makhov P et al. Musashi-2 (MSI2) regulates epidermal growth factor receptor (EGFR) expression and response to EGFR inhibitors in EGFR-mutated non-small cell lung cancer (NSCLC). Oncogenesis 10:29 (2021). PubMed: 33723247
- Mazzawi T et al. The Effects of Fecal Microbiota Transplantation on the Symptoms and the Duodenal Neurogenin 3, Musashi 1, and Enteroendocrine Cells in Patients With Diarrhea-Predominant Irritable Bowel Syndrome. Front Cell Infect Microbiol 11:524851 (2021). PubMed: 34055657
- Berenguer-Escuder C et al. Impaired mitochondrial-endoplasmic reticulum interaction and mitophagy in Miro1-mutant neurons in Parkinson's disease. Hum Mol Genet 29:1353-1364 (2020). PubMed: 32280985