Anti-Mus81 抗体 [MTA30 2G10/3] (ab14387)
![Western blot - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)](https://www.abcam.co.jp/ps/products/14/ab14387/Images/ab14387-21896-anti-mus81-antibody-mta30-2g10-3-western-blot.jpg "Western blot - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)")
Key features and details
- Mouse monoclonal [MTA30 2G10/3] to Mus81
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-Mus81 antibody [MTA30 2G10/3]
Mus81 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MTA30 2G10/3] to Mus81 -
由来種
Mouse -
アプリケーション
適用あり: WB, Flow Cytmore details -
種交差性
交差種: Human
非交差種: Mouse -
免疫原
Mus81 protein.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
from TCS -
ポリ/モノ
モノクローナル -
クローン名
MTA30 2G10/3 -
ミエローマ
Sp2/0-Ag14 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab14387の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (7) |
1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 66 kDa). Tested on both recombinant protein and whole cell extract.
|
| Flow Cyt |
Use 0.5µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 66 kDa). Tested on both recombinant protein and whole cell extract. |
|
Flow Cyt
Use 0.5µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks. -
組織特異性
Widely expressed. -
配列類似性
Belongs to the XPF family.
Contains 1 ERCC4 domain. -
発生段階
Expressed in S phase and G2 phase. -
細胞内局在
Nucleus > nucleolus. Recruited to foci of DNA damage in S-phase cells. - Information by UniProt
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参照データベース
- Entrez Gene: 80198 Human
- Omim: 606591 Human
- SwissProt: Q96NY9 Human
- Unigene: 288798 Human
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別名
- Crossover junction endonuclease MUS81 antibody
- FLJ21012 antibody
- FLJ44872 antibody
see all
画像
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Western blot - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)Anti-Mus81 antibody [MTA30 2G10/3] (ab14387) at 1/1000 dilution + HeLa cell lysate
Predicted band size: 66 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted? -
![Flow Cytometry - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)](https://www.abcam.co.jp/ps/products/14/ab14387/Images/ab14387-21895-anti-mus81-antibody-mta30-2g10-3-flow-cytometry.jpg)
Flow Cytometry - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)Overlay histogram showing HeLa cells stained with ab14387 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14387, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (45)
ab14387 は 45 報の論文で使用されています。
- Baxley RM et al. Bi-allelic MCM10 variants associated with immune dysfunction and cardiomyopathy cause telomere shortening. Nat Commun 12:1626 (2021). PubMed: 33712616
- Inoue N et al. The benzylisoquinoline alkaloids, berberine and coptisine, act against camptothecin-resistant topoisomerase I mutants. Sci Rep 11:7718 (2021). PubMed: 33833336
- Wu X & Wang B Abraxas suppresses DNA end resection and limits break-induced replication by controlling SLX4/MUS81 chromatin loading in response to TOP1 inhibitor-induced DNA damage. Nat Commun 12:4373 (2021). PubMed: 34272385
- Rødland GE et al. Differential Effects of Combined ATR/WEE1 Inhibition in Cancer Cells. Cancers (Basel) 13:N/A (2021). PubMed: 34359691
- Matos DA et al. ATR Protects the Genome against R Loops through a MUS81-Triggered Feedback Loop. Mol Cell 77:514-527.e4 (2020). PubMed: 31708417
