Anti-MURF2 抗体 (ab4387)
Key features and details
- Goat polyclonal to MURF2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-MURF2 antibody -
製品の詳細
Goat polyclonal to MURF2 -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Dog, Pig
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免疫原
Synthetic peptide:
HIFSFSWLNSLNE
, corresponding to C terminal amino acids 536-546 of Human MURF2. -
特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab4387 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 60 kDa. | |
| IHC-P | Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
May regulate gene expression and protein turnover in muscle cells. -
組織特異性
Highly expressed in muscle. Low-level expression in liver. -
配列類似性
Contains 1 B box-type zinc finger.
Contains 1 COS domain.
Contains 1 RING-type zinc finger. -
細胞内局在
Cytoplasm. Nucleus. Nuclear under atrophic conditions and upon mechanical signals. Localizes to the sarcomeric M-band in cardiomyocytes. Colocalizes in part with microtubules. - Information by UniProt
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参照データベース
- Entrez Gene: 84675 Human
- Entrez Gene: 381485 Mouse
- Entrez Gene: 365751 Rat
- Omim: 606469 Human
- SwissProt: Q9BYV6 Human
- SwissProt: Q5PQN5 Rat
- Unigene: 85524 Human
- Unigene: 35419 Mouse
see all -
別名
- MURF 2 antibody
- MURF-2 antibody
- MuRF2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MURF2 antibody (ab4387)
Immunohistochemical analysis (10 ug/ml) of Human Heart tissue labeling PBP2. Steamed antigen retrieval with citrate buffer pH 6.
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Western blot - Anti-MURF2 antibody (ab4387)Anti-MURF2 antibody (ab4387) at 0.1 µg/ml + Human heart lysate (in RIPA buffer) at 35 µg
Predicted band size: 60 kDa
データシートおよび資料
参考文献 (5)
ab4387 は 5 報の論文で使用されています。
- He J et al. MuRF2 regulates PPAR?1 activity to protect against diabetic cardiomyopathy and enhance weight gain induced by a high fat diet. Cardiovasc Diabetol 14:97 (2015). WB . PubMed: 26242235
- Cullup T et al. Recessive mutations in EPG5 cause Vici syndrome, a multisystem disorder with defective autophagy. Nat Genet 45:83-7 (2013). PubMed: 23222957
- Perera S et al. Developmental regulation of MURF E3 ubiquitin ligases in skeletal muscle. J Muscle Res Cell Motil 33:107-22 (2012). PubMed: 22426552
- Perera S et al. Developmental regulation of MURF ubiquitin ligases and autophagy proteins nbr1, p62/SQSTM1 and LC3 during cardiac myofibril assembly and turnover. Dev Biol 351:46-61 (2011). WB ; Mouse . PubMed: 21185285
- Willis MS et al. Muscle ring finger 1, but not muscle ring finger 2, regulates cardiac hypertrophy in vivo. Circ Res 100:456-9 (2007). WB ; Mouse . PubMed: 17272810
