Anti-Munc18-1 抗体 [EPR4850] - BSA and Azide free (ab248059)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4850] to Munc18-1 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Munc18-1 antibody [EPR4850] - BSA and Azide free
Munc18-1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4850] to Munc18-1 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
ab248059 is the carrier-free version of ab124920.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4850 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248059の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
May participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions. -
組織特異性
Brain and spinal cord. Highly enriched in axons. -
関連疾患
Defects in STXBP1 are the cause of epileptic encephalopathy early infantile type 4 (EIEE4) [MIM:612164]. Affected individuals have neonatal or infantile onset of seizures, suppression-burst pattern on EEG, profound mental retardation, and MRI evidence of hypomyelination. -
配列類似性
Belongs to the STXBP/unc-18/SEC1 family. -
細胞内局在
Cytoplasm. Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 6812 Human
- Entrez Gene: 20910 Mouse
- Entrez Gene: 25558 Rat
- Omim: 602926 Human
- SwissProt: P61764 Human
- SwissProt: O08599 Mouse
- SwissProt: P61765 Rat
- Unigene: 288229 Human
see all -
別名
- FLJ37475 antibody
- Munc 18 1 antibody
- Munc 18a antibody
see all
画像
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All lanes : Anti-Munc18-1 antibody [EPR4850] (ab124920) at 1/10000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Y79 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 68 kDaThis data was developed using ab124920, the same antibody clone in a different buffer formulation.
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This data was developed using ab124920, the same antibody clone in a different buffer formulation.ab124920, at 1/250 dilution, staining Munc18 in paraffin embedded Human brain tissue by immunohistochemistry Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab248059 は論文での使用が確認できていません。